乳胞素对胶质瘤C6细胞体内增殖的抑制作用  

Inhibitory effect of lactacystin on proliferation of glioma C6 cells in vivo

作　　者：王海峰[1] 陈勃[1] 钟加滕[2] 项喜艳[2] 李文臣[1] 罗毅男[1] 葛鹏飞[1] 

机构地区：[1]吉林大学第一医院神经外科,吉林长春130021 [2]吉林大学白求恩医学院病理生理学教研室,吉林长春130021

出　　处：《吉林大学学报（医学版）》2011年第5期801-804,971,共4页Journal of Jilin University：Medicine Edition

基　　金：中国博士后基金项目资助课题(20080440422);吉林省科技厅科研基金资助课题(W200705460)

摘　　要：目的:在体外实验蛋白酶体抑制剂乳胞素(LAC)可以抑制神经胶质瘤C6细胞增殖的基础上,进一步在裸鼠体内观察乳胞素对C6细胞增殖率的影响,探讨乳胞素抑制胶质瘤细胞增殖率的可能机制。方法:体外培养C6细胞,选择处于对数生长期的细胞接种于裸鼠背部皮下,连续7 d腹腔注射给予乳胞素,实验分为模型组(Model)、乳胞素0.5μg/20 g组、乳胞素1μg/20 g组及乳胞素5μg/20 g组,每组3只裸鼠,每天观察小鼠一般状态(饮食、大小便、毛色、活动情况等);测量与计算不同时间点肿瘤体积,RT-PCR方法检测各组细胞中Bax、bcl-2和caspase-3 mRNA表达水平。结果:与模型组比较,乳胞素0.5μg/20 g组、乳胞素1μg/20 g组和乳胞素5μg/20 g组肿瘤体积明显减小(P<0.05),Bax/bcl-2和caspase-3 mRNA表达水平明显升高(P<0.05)。不同浓度乳胞素组肿瘤体积无明显差异,不同浓度乳胞素组Bax/bcl-2 mRNA水平及caspase-3 mRNA水平无明显差异。结论:乳胞素在动物体内能明显抑制C6细胞的增殖率,其作用机制可能是增加C6细胞线粒体途径的凋亡发挥抑制其增殖的作用。Objective To observe the effect of lactacystin（LAC） on the proliferation rate of C6 cells in vivo on the basis of inhibitory effect of LAC on C6 cells in vitro and to discuss the possible mechanism of LAC inhibiting the proliferation rate of C6 cells.Methods The C6 cells were cultivated in vitro,and the cells in the logarithmic growth phase were selected and were inoculated in nude mice subcutaneously.All mice were given LAC for 7 d.They were divided into model group,LAC 0.5 μg / 20 g group,LAC 1 μg / 20 g group,and the LAC 5 μg / 20 g group.There were three nude mice in each group.The general condition of mice（food,bathroom,coat color,activities,etc.） was observed;the tumor volumes at different time points were measured and calculated.The Bax,bcl-2 and caspase-3 mRNA expression levels were detected by RT-PCR method.Results Compared with model group,the tumor volumes in LAC 0.5 μg / 20 g group,LAC 1 μg / 20 g Group,and LAC 5 μg / 20 g group were significantly reduced（P0.05）;the Bax/bcl-2 and caspase-3 mRNA expression levels were increased significantly（P0.05）.There was not statistically significant between different doses of LAC in tumor volume,level of Bax/bcl-2 mRNA and level of caspase-3 mRNA,respectively.Conclusion LAC in animals can significantly inhibit the proliferation rate of C6 cells and its mechanism may be that it increases apoptosis of mitochondrial pathway in C6 cells to inhibit the proliferation.

关 键 词：乳胞素 凋亡 BALBC 胶质瘤 

分 类 号：R739.41[医药卫生—肿瘤]