特定寡核苷酸对大鼠实验性牙移动的影响  

Influence of specific oligodeoxynucleotides on experimental tooth movement in rats

作　　者：王静[1,2] 孙新华[1] 冯志远[1] 申玉芹[3] 

机构地区：[1]吉林大学口腔医院正畸科,吉林长春130021 [2]河北省沧州市人民医院正畸科,河北沧州061000 [3]吉林大学口腔医院牙周病科,吉林长春130021

出　　处：《吉林大学学报（医学版）》2011年第5期812-816,I0001,共6页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅自然科学基金资助课题(201015203)

摘　　要：目的:探讨特定寡核苷酸(ODN)对大鼠实验性牙移动的影响,阐明其在牙周组织改建中的作用特点。方法:40只雄性Wistar大鼠,随机分为实验组(n=20)和对照组(n=20),建立大鼠牙移动模型。加力当天于实验组大鼠两侧上颌第一磨牙颊侧牙龈黏膜下各注射50μL特定ODN(20 mg.L-1),对照组则于相同部位注射等量的PBS溶液,每3 d给药1次,取3、7、14、21和28 d 5个时间点各处死4只大鼠。建模前和处死前取模型测定牙移动距离,经HE染色观察牙周组织变化并计数破骨细胞(OC)。结果:大鼠牙移动距离随时间变化逐渐增大,除3 d组外实验组大鼠第一磨牙牙移动距离均小于对照组(P<0.05或P<0.01);HE染色观察,实验组大鼠第一磨牙压力侧牙槽骨吸收程度轻于对照组,OC数少于对照组,3、7和14 d时2组间差异具有统计学意义(P<0.05或P<0.01)。结论:特定ODN可减少压力侧牙槽骨表面OC的数量从而抑制实验性牙齿移动,对正畸牙周组织的改建产生影响。Objective To study the influence of specific oligodeoxynucleotides（ODN） on the experimental tooth movement in rats and to clarify its role in the reconstruction of periodontal tissues.Methods Fourty male Wistar rats were randomly divided into experiment group（n= 20） and control group（n= 20）.The tooth movement models were set up.Then 50μL specific ODN（20 mg·L-1） was injected into both sides of the buccal gingival mucosa adjacent to the first upper molar in experiment group,the rats in control group were injected by the same amount of PBS in the sam place,once every three days.4 rats were killed after 3,7,14,21 and 28 d in each group.Then the distance of tooth movement was measured before the models were made and before the rats was killed,and the changes of periodontal tissues were observed by HE staining and the number of osteoclasts was counted.Results The distance of tooth movement in rats were gradually increased.Except for 3 d group,the distances in experiment group were shorter than those in control group（P0.05 or P0.01）.HE staining showed that the Absorption on pressure side of the first molar alveolar bone and the number of osteoclasts in experiment group were less than those in control group,especially on the days of 3,7 and 14（P0.05 or P0.01）.Conclusion The specific ODN can inhibit the tooth movement by reducing the number of OC on the pressure side of alveolar bone,so the specific ODN can influence the reconstruction of periodontal tissues.

关 键 词：寡核苷酸 正畸 牙移动 牙周组织 

分 类 号：R783.5[医药卫生—口腔医学]