鲨鱼软骨粉对S_(180)荷瘤小鼠免疫细胞调节功能的影响  

Effect of shark cartilage powder on immune cells regulatory functions in S_(180) bearing mice

作　　者：张秀娟[1,2] 刘思含[1,2] 严松[3] 季宇彬[1,2] 

机构地区：[1]哈尔滨商业大学生命科学与环境科学研究中心生物安全与评价研究所,黑龙江哈尔滨150076 [2]国家教育部抗肿瘤天然药物教育部工程研究中心,黑龙江哈尔滨150076 [3]黑龙江省农业科学院食品加工研究所,黑龙江哈尔滨150086

出　　处：《中国海洋药物》2011年第5期29-33,共5页Chinese Journal of Marine Drugs

基　　金：黑龙江省自然科学基金项目(D201043)

摘　　要：目的研究鲨鱼软骨粉(SCP)对S180荷瘤小鼠免疫细胞功能的影响。方法鲨鱼软骨粉300、200、100mg·kg-1小鼠灌胃给药10 d。MTT法检测其对S180荷瘤小鼠脾淋巴细胞增殖功能的影响;流式细胞仪检测T淋巴细胞亚群的变化;ELISA法测定荷瘤小鼠血清中细胞因子TNF-α、IFN-γ含量。结果 SCP能提高小鼠脾淋巴细胞的增殖活性;不同程度地提高小鼠脾细胞CD4+细胞数,增加CD4+/CD8+细胞比值;明显提高小鼠巨噬细胞的吞噬活性;促进外周血清中TNF-α和IFN-γ的分泌水平。结论 SCP能提高T细胞介导的细胞免疫应答,提高巨噬细胞调节免疫应答的功能,促进TNF-α、IFN-γ等细胞因子的分泌,从而发挥其抗肿瘤作用。Objective To study the effect of shark cartilage powder（SCP） on immune cells regulatory functions in S180 bearing mice.Methods Shark cartilage powder at doses of 300,200,100 mg·kg-1,ig.for 10 days.The MTT assay was used to determine the effect of SCP on spleen lymphocytes proliferation.The rate of CD＋4/CD＋8 cell was adopted by flow cytometry and the levels of TNF-α and IFN-γ were measured by ELISA method.Results The SCP could increase the proliferation of spleen lymphocytes and the number of CD＋4 cells as well as the ratio of CD＋4/CD＋8 cell significantly.SCP could raise the levels of TNF-α and IFN-γ.Conclusion SCP could enhance the immunologic function,promote the secretions of TNF-α and IFN-γ,and could be applied in anti-tumor therapy.

关 键 词：鲨鱼 软骨 免疫功能 脾淋巴细胞 细胞因子 

分 类 号：R931.74[医药卫生—生药学] R965[医药卫生—药学]