光合细菌对2,4,6-三氯苯酚的降解特性研究  

Biodegradation characteristics of 2,4,6-trichlorophenol by photosynthetic bacteria

作　　者：姚斌[1] 金赞芳[1] 胡忠策[1] 金琼[1] 潘志彦[1] 

机构地区：[1]浙江工业大学生物与环境工程学院,浙江杭州310032

出　　处：《中国环境科学》2011年第10期1669-1675,共7页China Environmental Science

基　　金：国家科技重大专项(2008ZX07101-006);国家自然科学基金资助项目(40803027);浙江省环境污染控制技术研究重点实验室项目(2010-09)

摘　　要：研究了混合光合细菌PSB-DR在不同光照、接种量和pH值下对2,4,6-三氯苯酚(2,4,6-TCP)的生物降解特性,确定了PSB-DR生物降解2,4,6-TCP的优化控制条件.结果表明,光照培养下,接种量30%,初始pH值7.0时2,4,6-TCP降解效率最高.在此条件下,50mg/L的2,4,6-TCP经5d后降解率达到82.3%.培养基中醋酸钠的加入对2,4,6-TCP降解有明显的抑制作用.PSB-DR静息细胞对2,4,6-TCP的降解符合高浓度底物抑制的酶促反应类型,其降解动力学参数rmax=1.746h-1,Km=38.333mg/L,Ki=260.87mg/L.Degradation of 2,4,6-trichlorophenol（2,4,6-TCP） by mixed cultures photosynthetic bacteria PSB-DR at different illumination,inoculum density and initial pH were investigated.The degradation conditions were optimized to be incubation with an inoculum density of 30% and initial pH 7.0 under light.About 82.3% of 2,4,6-TCP with initial concentration of 50mg/L was removed after 5d incubation under the condition.The addition of sodium acetate into the substrate greatly suppressed 2,4,6-TCP biodegradation.The degradation of 2,4,6-TCP could be well described by the enzymatic reaction of high concentration inhibition,with the maximum substrate utilization rate 1.746h-1,Michaelis-Menten constant 38.333mg/L and inhibitory constant 260.87mg/L,respectively.

关 键 词：光合细菌 生物降解 2 4 6-三氯苯酚 降解动力学 

分 类 号：X172[环境科学与工程—环境科学]