薄荷素油特征图谱的研究及多组分含量测定  

Study on specific chromatogram and determination of chemical components in peppermint oil

作　　者：陈在敏[1] 

机构地区：[1]福建省药品检验所,福州350001

出　　处：《药物分析杂志》2011年第10期1957-1960,共4页Chinese Journal of Pharmaceutical Analysis

基　　金：国家科技支撑计划子课题(2009ZX09308-001-3)

摘　　要：目的:建立薄荷素油的气相特征图谱共有模式,研究薄荷素油的化学成分。建立同时测定薄荷素油中α-蒎烯,柠檬烯、薄荷酮、薄荷醇、胡薄荷酮、薄荷醋酸酯6种成分含量的气相色谱法。方法:采用GC-MS对薄荷素油中的共有成分进行鉴定。含量测定采用GC内标法,色谱柱:HP-5毛细管柱(30 m×0.32 mm×0.25μm),以1-辛醇为内标。结果:薄荷素油中的15种共有成分得到鉴定,建立了薄荷素油含11个主要共有峰的气相特征图谱。含量测定中6种主要成分得到良好的分离,在测定范围内的线性关系良好,平均回收率在97%以上。结论:特征图谱结合多组分的含量测定,能更好地控制薄荷素油的质量。Objective：To set up the mutual mode of GC specific chromatograms of peppermint oil.To develop a GC method for simultaneous determination of α-pinene,limonene,menthone,menthol,pulegone,menthyl acetate in peppermint oil.Method：GC-MS was applied for the identification of the mutual components in peppermint oil.GC was performed on HP-5 capillary column（30 m×0.32 mm×0.25 μm）and 1-octanol used as an internal standard.Result：15 common components were identified by GC-MS in peppermint oil.The GC specific chromatogram of peppermint oil with 11 common characteristic peaks was established.Six main components were finely separated and showed good linearity in the concentration range of detection,the added sample recovery rate was above 97%.Conclusion：The combination of specific chromatograms and GC determination is capable of effectively controlling of the quality of peppermint oil.

关 键 词：薄荷素油 特征图谱 气相色谱-质谱联用 α蒎烯 柠檬烯 薄荷酮 藻荷醇 胡薄荷酮 薄荷醋酸酯 含量测定 

分 类 号：R917[医药卫生—药物分析学]