苦荞的HPLC指纹图谱分析  

HPLC fingerprint of buckwheat

作　　者：宾婕[1] 刘洁[1] 张春勇[1] 陈克嶙[2] 陈丹 周劲 郭荣富[2] 郭亚东[1] 

机构地区：[1]昆明医学院药学院,昆明650500 [2]云南农业大学动物科技学院,昆明650201 [3]云南侨通包装印刷有限公司,昆明657000

出　　处：《药物分析杂志》2011年第10期1961-1964,共4页Chinese Journal of Pharmaceutical Analysis

基　　金：云南侨通包装印刷有限公司资助项目(kx140564)

摘　　要：目的:建立苦荞的HPLC指纹图谱分析方法。方法:采用岛津ODS柱(4.6 mm×150 mm,5μm),流动相为乙腈–水(含2%的醋酸),梯度洗脱,检测波长280 nm,流速:1 mL.min-1。采用《中药色谱指纹图谱相似度评价系统(2004A)版》,计算不同产地苦荞样品的HPLC指纹图谱的整体相似度。结果:共有12个共有峰,16批被测样品的色谱指纹图谱的整体相似度在0.927～0.999。结论:该指纹图谱检测方法简便、重现性好,可作为苦荞药材质量控制的重要方法。Objective：To establish the analysis method of fingerprint for buckwheat by HPLC.Methods：A column of Shimadzu ODS（4.6 mm×150 mm,5 μm）was used.The mobile phase consisted of acetonitrile-water（containing 2% acetic acid） with gradient elution.The detective wavelength was at 280 nm,and the flow rate was 1.0 mL·min^-1.The similarity of different origin buckwheat sample were compared by Similarity Evaluation System for Chromatographic Fingerprint of CMM Version 2004A.Results：The fingerprint consisted of 12 common peaks.and the similarity was in the range of 0.927-0.999.Conclusion：This method is simple,accurate with good reproducibility,and can be used specifically for the quality control of buckwheat.

关 键 词：苦荞 指纹图谱 高效液相色谱 

分 类 号：R917[医药卫生—药物分析学]