天然苄基异喹啉类抗肿瘤生物碱的筛选  

Screen for natural benzylisoquinoline alkaloids against tumor

作　　者：张正付[1] 郭莹[2] 魏雄辉[1] 

机构地区：[1]北京大学化学与分子工程学院,北京100871 [2]北京大学医学部,北京100083

出　　处：《中国中药杂志》2011年第19期2684-2688,共5页China Journal of Chinese Materia Medica

摘　　要：目的:寻找具有抗肿瘤活性的天然苄基异喹啉类生物碱。方法:本实验建立以DNA为靶标,运用离心超滤和HPLC联用技术,从中药中筛选可能具有抗肿瘤活性的苄基异喹啉类生物碱活性成分的方法;并利用体外肿瘤细胞实验,对筛选出的化合物进行抗肿瘤活性验证和评价。结果:从博落回等3种中药中发现并鉴定了9种化合物与DNA有相互作用,有良好的细胞增殖抑制活性。结论:建立起来的方法能用于中药中具有抗肿瘤活性的天然苄基异喹啉类生物碱的筛选。Objective： To search for natural benzylisoquinoline alkaloids against tumor. Method： In this study, taken DNA as target, a method was developed for screening of natural benzylisoquinoline alkaloids against tumor from traditional Chinese medicine by the use of centrifugal ultrafiltration combined with HPLC technology. The anti-tumor activity for the compounds screened was evaluated in.vitro. Result： Nine compounds interacted with DNA were discovered and identified from Macleaya cordata, Chelidonium majus, Coptis chinensis, and the proliferation of four types of human solid cancer cell lines was markedly inhibited by these compounds. Conclusion： The developed method was considered to be suitable for screening of natural benzylisoquinoline alkaloids against tumor from traditional Chinese medicine.

关 键 词：苄基异喹啉类生物碱 DNA 抗肿瘤 筛选 

分 类 号：TQ461[化学工程—制药化工] R284[医药卫生—中药学]