NMDA受体介导的eEPSC在皮质发育障碍动物模型海马CA1区的改变  

Changes of NMDA receptors-mediated eEPSC in hippocampal CA1 region of the DCDs rat models

作　　者：张建刚[1] 宋延波[2] 张毅[1] 贺兴[1] 冯飞[1] 晏勇[1] 

机构地区：[1]重庆医科大学第一附属医院神经内科,重庆400016 [2]重庆医科大学第一附属医院肿瘤科,重庆400016

出　　处：《癫痫与神经电生理学杂志》2011年第5期257-262,共6页Journal of Epileptology and Electroneurophysiology(China)

摘　　要：目的：研究皮质发育障碍（DCD）大鼠模型海马CA1区N-甲基-D-门冬氨酸（NMDA）受体及α-氨基-3-羧基5甲基异恶唑-4-丙酸（AmPA）受体介导的兴奋性突触后电流（eEPSC）的变化，探讨DCD大鼠模型的致痫机制。方法：选取出生10～20dDCD幼鼠模型和正常对照组，应用可视法脑片膜片钳记录方法，记录大鼠海马CA1区锥体神经元的NMDA受体及AmPA受体介导的eEPSC幅度及衰减时间常数。结果：DCD模型组与正常对照组相比，NMDA受体介导的eEPSC的幅度有明显增高r（119．54±10．97）pAVS（83．69±10．23）pA；P〈0．053；衰减时间常数明显延长[（154．59±3．92）VS（117．18±4．04）；P〈0．05]。而AmPA受体介导的eEPSC的幅度[（139．99±23．41）pAVS（135．50±26．44）pA；P〉0．05]及衰减时间常数[（47．23±2．28）VS（48．68±2．20）；P〉0．053无明显改变。结论：NMDA受体介导的异常突触后反应在DCD的致痫机制方面起到重要的作用。Objective：To observe the changes of N-methyl-D-aspartate receptor （NMDAR） and α- amino-3 hydroxy-5-methyl-4-isox-Azoleprop-ionic acid receptor （AInPAR） mediated evoked excitatory post synaptic currents（eEPSC） in hippocampal CA1 region of the DCD rat models. To investigate the epileptogenic mechanism in rat models of the disorder of cortical development（DCD）. Methods： The amplitude and decay time constant of NMDAR and AmPAR mediated eEPSC in hippocampal CA1 region py- ramidal neurons of DCD rat models were observed using visual patch clamp whole-cell recording technique when the offspring rats were P10d-P20d. Results： Compared with the control group, the amplitude of NMDAR mediated eEPSC in DCD rat models significantly increased[（119. 54±10.97） pA vs （83. 69± 10.23） pA;P〈0.05]. The decay time constant of NMDAR mediated eEPSC in DCD rat models was significantly delayed[（154.59±3.92） vs （117.18±4.04） ;P〈0. 051. The amplitude and decay time constant of AmPAR mediated eEPSC had no difference between the DCD group and the control group. [（139.99±23.41） pAvs （135.50±26.44） pA;P〉0. 05][（47. 23±2. 28） vs （48.68±2.20）;P〉0. 051. Conclusion： The NMDAR mediated hyper excitable synaptic responses could play an important role in epileptogenic mechanism of DCDs rat models.

关 键 词：皮质发育障碍DCD) 海马 诱发的兴奋性突触后电流(eEPSC) 

分 类 号：R741.044[医药卫生—神经病学与精神病学]