中药复方药物血清对FADD和MCL-1基因在人卵巢癌细胞株SKOV3中表达的影响  

Effect of Chinese Herbal Compound Drug Serum on the Expression of FADD and MCL-1 Gene in Ovarian Cancer Cell Line SKOV3

作　　者：王熙月[1] 韩凤娟[1] 于燕[1] 吴效科[1] 侯丽辉[1] 

机构地区：[1]黑龙江中医药大学附属第一医院妇产科,哈尔滨150040

出　　处：《医学研究杂志》2011年第9期41-44,共4页Journal of Medical Research

基　　金：教育部春晖计划基金资助项目(Z2007-1-15001);黑龙江省自然科学基金面上项目(D200816);黑龙江中医药大学杰出人才培育基金资助项目(200703)

摘　　要：目的研究FADD和MCL-1基因在中药复方理冲生髓饮诱导人卵巢癌细胞株SKOV3细胞凋亡中的作用。方法 Wistar大鼠随机分组,灌胃给药,采血制备含药血清。提取生理盐水组及理冲生髓饮组肿瘤细胞mRNA,制备DNA探针并分别用Cy3和Cy5两种荧光染料标记,与人细胞凋亡寡核苷酸微阵列芯片进行杂交,经扫描、分析,得出药物作用后表达有差异的基因。结果共筛选出显著表达差异基因66种,其中20种基因表达水平上调,46种基因表达水平下调。结论理冲生髓饮能够抑制细胞增殖并诱导其凋亡,其细胞凋亡机制与上调FADD基因和下调MCL-1基因有关。Objective To study the effect of the FADD and MCL - 1 gene in apoptosis of human ovarian cancer cell line SKOV3 in- duced by Liehongshengsui drink with the cDNA chip technique. Methods Wistar rats were divided into two group randomly and treated with drugs,then the drug serum samples were prepared, mRNA of tumor ceils in physiological saline group and Lichongshengsui drink group were extracted. DNA probe was prepared and marked with two kinds of fluorescent dyes ： Cy3 and Cy5, respectively, hybridized with human apoptosis oligonucleotide microarray, then the differentially expressed genes were obtained after scanning and analysis. Results Sixty - six differentially expressed genes were screened out, including 20 kinds of up regulation genes and 46 kinds of down regulation genes. Conclusion Lichongshengsui drink can inhibit cell proliferation and induce the apoptosis of ovarian cancer cell line SKOV3 ,which may be related to up - regulation FADD gene and down - regulation MCL - 1 gene.

关 键 词：理冲生髓饮 药物血清 细胞凋亡 卵巢癌 基因芯片 SKOV3 

分 类 号：R733[医药卫生—肿瘤]