HIF-1α、VEGF在疣状胃炎中的表达及意义  

Expression and Significance of HIF-1α,VEGF in Errucous Gastritis

作　　者：卜煜锋[1,2] 张晓海[1,2] 张洪涛[1,2] 桑旭东[1,2] 高丽琴[1,2] 褚利娟[1,2] 施惠娟[1,2] 

机构地区：[1]浙江省皮肤病防治研究所 [2]浙江省武康疗养院,德清313200

出　　处：《医学研究杂志》2011年第9期141-143,共3页Journal of Medical Research

摘　　要：目的观察疣状胃炎胃黏膜组织中HIF-1α、VEGF的表达,探讨疣状胃炎的可能发生机制及其与癌前病变的关系。方法经胃镜及病理确诊的疣状胃炎94例,其中非成熟型46例,,成熟型48例,在隆起处近中心部位及距隆起病变边缘5cm的平坦胃黏膜处各取胃黏膜组织2块;设同期经胃镜和病理均确诊为慢性浅表性胃炎(慢性轻度、非活动型、Hp阴性)30例作为对照,取胃窦小弯处取胃黏膜组织2块;14C标记尿素呼气试验检测患者幽门螺旋杆菌(Hp)感染情况,免疫组化EnVision二步法检测患者胃黏膜组织中HIF-1α、VEGF的表达情况。结果疣状胃炎患者疣状病灶部位组织HIF-1α、VEGF的表达均较疣状旁5cm平坦处明显增高(P<0.01)。非成熟型和成熟型疣状胃炎患者其胃黏膜疣状病灶部位HIF-1α、VEGF的表达均较对照组明显增高(P<0.01,P<0.05);但两组之间HIF-1α、VEGF的表达差异无显著性(P>0.05)。疣状胃炎患者胃黏膜组织HIF-1α与VEGF的表达水平呈明显的正相关(r=0.729,P<0.01)。结论 HIF-1α及其下游因子VEGF在疣状胃炎患者疣状隆起病灶部位表达增强,可能参与了疣状胃炎的形成和发展。Objective To observe the expression of HIF -1α, VEGF in gastric mucosa of verrucous gastritis, to explore the possihle pathogenesis of verrucous gastritis. Methods All 94 cases patients in accordance with the endoscopic diagnostic criteria of verrucous gas- tritis were classified on the basis of endoscopic features,of which 46 cases belonged to immature, and 48 cases mature. From the mucosal protrusion and the flat gastric mucosa,Scm from the edge of elevated lesion,two pieces of gastric mucosal specimens were obtained. Moreo- ver, as the control,30 cases of chronic superficial gastritis were diagnosed through endoscope and pathology,in which two pieces of gastric mucosal specimens in antral lesser curvature were obtained. Hp was detected with 14C - labeled urea breath test. HIF -1α, VEGF expres- sions of gastric mucosa were detected with immunohistochemical EnVision two - step test. Results The expressions of HIF -1α,VEGF in focus adjacent tissues of verrucous gastritis were obviously enhanced than those of the fiat of focus adjacent tissues（ P 〈 0.01 ）. The expres- sions of HIF -1α,VEGF in focus adjacent tissues of immature and mature verrucous gastritis were obviously enhanced than those in the control （ P 〈 0.01, P 〈 0.05 ）. But the difference between the two groups was insignificant （ P 〉 0.05 ）. The expressions of HIF -1α in focus tissues of verrucous gastritis was obviously positive - related with the expressions of VEGF （ r = 0. 729,P 〈 0. 01 ）. Conclusion There is enhanced expression of HIF -1α and its downstream factor VEGF in mucosal raised focuses of verrucous gastritis which may take part in the formation and development of verrucous gastritis.

关 键 词：疣状胃炎 缺氧诱导因子-1α亚基 血管内皮生长因子 幽门螺杆菌 

分 类 号：R573.305[医药卫生—消化系统]