雌激素对自发性高血压大鼠颅内动脉血管壁TNF-α和MMP-9表达的影响  

Role of estrogen on expression of TNF-α and MMP-9 in intracranial arterial vascular wall of spontaneous hypertensive rats

作　　者：刘安民[1] 刘军[2] 蔡望青[1] 胡震[1] 郑眉光[1] 

机构地区：[1]中山大学孙逸仙纪念医院神经外科,广东广州510120 [2]中山大学孙逸仙纪念医院神经科,广东广州510120

出　　处：《中国病理生理杂志》2011年第9期1731-1735,共5页Chinese Journal of Pathophysiology

基　　金：广东省医学科研基金资助项目(No.A2008185)

摘　　要：目的:探讨雌激素对颅内动脉血管壁炎症反应和血管重塑的可能作用。方法:雌性自发性高血压大鼠(SHR)32只,随机分为4组:自发性高血压模型对照组、卵巢切除去势组、去势后雌激素替代组和卵巢切除去势对照组,每组8只。放射免疫法检测各组静脉血的雌激素水平,获取标本后光镜下观察血管壁的病理变化,运用Western blotting检测颅内动脉Willis环血管壁组织TNF-α和MMP-9蛋白的含量。结果:自发性高血压模型对照组雌激素水平明显高于卵巢切除去势组(P<0.01);卵巢切除去势后雌二醇替代组的雌激素水平较卵巢切除去势对照组明显增高(P<0.01)。所有大鼠均未见进展期动脉瘤。在自发性高血压模型对照组中未见动脉瘤样改变,在卵巢切除去势组、卵巢切除去势对照组和卵巢去势后雌激素替代组中分别发现2个、3个和1个早期动脉瘤样改变。自发性高血压模型对照组颅内动脉血管壁TNF-α和MMP-9的含量明显低于卵巢切除去势组,雌二醇替代治疗组颅内动脉血管壁的TNF-α和MMP-9表达明显低于卵巢去势对照组(P<0.01)。结论:雌激素能通过抑制颅内动脉的炎性反应,降低血管壁MMP-9的表达,影响颅内动脉的血管重塑。AIM： To study the effects of estrogen on the inflammatory response and vascular remodeling of intracranial artery in rats.METHODS： Thirty-two female spontaneous hypertensive rats（SHR） were randomized into 4 groups： spontaneous hypertensive group（sham-operated）,ovariectomized group,ovariectomized＋17 beta-estradial group and ovariectomized＋vehicle group（8 rats in each group）.On day 14,estradiol was detected by radioimmunoassay.The pathological changes were observed under light microscope.The protein expression of tumor necrosis factor alpha（TNF-α） and matrix metalloproteinase-9（MMP-9） in vascular wall of Willis circle was detected by Western blotting.RESULTS： The estrogen level was lower in ovariectomized group than that in sham-operated group（P0.01）.The estrogen level was higher in ovariectomized rats treated with 17 beta-estradial than that in ovariectomized rats treated with vehicle（P0.01）.Advanced aneurysm was not found in all groups.Early aneurysmal change was not found in sham-operated group.Early aneurysmal changes in some rats were observed in ovariectomized group（2 rats）,ovariectomized＋vehicle group（3 rats） and ovariectomized＋17 beta-estradial group（1 rat）.The protein levels of TNF-α and MMP-9 in the vascular wall of Willis circle in sham-operated group were lower than that in ovariectomized group（P0.01）.Additionally,the protein levels of TNF-α and MMP-9 in the vascular wall of Willis circle of ovariectomized rats treated with 17 beta-estradial were lower than those of ovariectomized rats treated with the vehicle（P0.01）.CONCLUSION： Estrogen can influence the vascular remodeling of intracranial artery by inhibiting the inflammatory response and degradating MMP-9 in the vascular wall.

关 键 词：颅内动脉 雌激素类 血管重塑 炎症 

分 类 号：R363[医药卫生—病理学]