内蒙古荒漠生物结皮细菌的分离、鉴定  

ISOLATION,IDENTIFICATION OF BACTERIA FROM BIOLOGICAL SOIL CRUST OF INNER MONGOLIA DESERTS

作　　者：刘柯澜[1] 冯福应[1] 

机构地区：[1]内蒙古农业大学生命科学学院应用与环境微生物实验研究所,呼和浩特010018

出　　处：《内蒙古农业大学学报（自然科学版）》2011年第3期203-206,共4页Journal of Inner Mongolia Agricultural University(Natural Science Edition)

基　　金：国家自然科学基金项目(30860015);教育部春晖项目(Z2007-1-01017);内蒙古自治区重大自然科学基金项目(2009ZD03)

摘　　要：采用PGY-BG11生物结皮细菌培养基,运用传统培养方法从内蒙古浑善达克沙地、毛乌素沙地的生物结皮中分别分离到21株和12株细菌。采用16Sr-DNA测序分析方法进行菌种鉴定,得出两地的优势菌均为链霉菌属,并根据RDP在线程序Classifier浑善达克沙地、毛乌素沙地生物结皮可培养细菌归类。与采用分子生物学方法研究相同采样地生物结皮细菌群落结构的结论相比较,得出不仅优势菌不同,而且通过纯培养获得菌株所占类群的数量也少于采用分子生物学方法研究生物结皮细菌群落所属的类群。PGY-BG11 Biological Soil Crusts Medium were used to isolate and screen microorganisms,and 21 strains and 12 strains were isolated from sample of biological soil crust of Hunshandake sandy land and Maowusu sandy land respectively.To identify these bacterium adopted the method of 16Sr-DNA sequence appraisal and concluded that both dominant bacteria were Streptomyces.We use RDP online program Classifier to classify these bacterium accorded with their sampling site.When compared with the result of the same sampling site of bacterial community which studied by molecular biotechnology,we found that not only the dominant bacteria was different from each other,but also the main component obtained from culture method was less than the main component of molecular biotechnology.

关 键 词：浑善达克沙地 毛乌素沙地 生物结皮 16Sr-DNA鉴定 优势菌 

分 类 号：Q93-331[生物学—微生物学]