Grb2-相关蛋白2参与RANKL诱导的乳腺癌细胞MDA-MB-231迁移的机制研究  

Grb2 was involved in RANKL-induced breast cancer cell MDA-MB-231 migration

作　　者：张凌云[1] 曲秀娟[1] 刘云鹏[1] 侯科佐[1] 

机构地区：[1]中国医科大学附属第一医院,110001

出　　处：《山西医药杂志（上半月）》2011年第10期957-959,共3页Shanxi Medical Journal

基　　金：国家青年科学基金(30700807);辽宁省教育厅基金(2010225032);辽宁省教育厅重点实验室项目(2008S246)

摘　　要：目的探讨Grb2-相关蛋白2(Gab2)在核因子-κB受体活化因子配体(RANKL)诱导的乳腺癌细胞MDA-MB-213迁移中的作用。方法流式细胞术检测MDA-MB-231细胞表面RANK的表达。Transwell法测定RANKL刺激后细胞迁移能力的改变。免疫沉降及Western-blot检测RANKL刺激后p-Tyr-Gab2和Gab2的表达。结果 MDA-MB-231细胞表达RANK蛋白,RANKL诱导MDA-MB-231细胞迁移能力增强。RANKL刺激后MDA-MB-231细胞p-Tyr-Gab2表达一过性升高。结论 Gab2参与RANKL诱导的乳腺癌细胞MDA-MB-231迁移。Objective To explore the role of Gab2 in regulation of breast cancer cell migration by RANKL.Methods Detection of cell-surface expression of RANK protein was performed by nuorescence-activated cell sorting（FACS）.Transwell assayed the migration of breast cancer cell.Immunoprecipitation and Western blot was used to assay the expression of p-Tyr-Gab2,Gab2 and actin.Results Fluorescence-activated cell sorting showed that RANK is expressed in human breast cancer cell line MDA-MB-231,and RANKL significantly increased the migration of breast cancer cells,which was blocked by decoy receptor OPG.The expression of p-Tyr-Gab2 increased at 5 minutes after RANKL stimulated.Conclusion Gab2 was involved in RANKL induced breast cancer cell MDA-MB-231 migration.

关 键 词：乳腺肿瘤 细胞运动 核因子-ΚB受体活化因子配体 

分 类 号：R737.9[医药卫生—肿瘤]