慢性乙型病毒性肝炎中Bcl-2及Bax的表达与T、B淋巴细胞数量变化的关系  

Bcl-2 and Bax expression and its relation with number of T and B lymphocytes in patients with chronic hepatitis B

作　　者：郭芳[1] 陈秋月[1] 周英琼[1] 林静[1] 李美琼[1] 侯巧燕[1] 

机构地区：[1]桂林医学院附院病理科病理教研室,广西桂林541001

出　　处：《现代医药卫生》2011年第20期3047-3049,共3页Journal of Modern Medicine & Health

基　　金：广西教育科学"十一五"规划B类课题(课题编号:2008B36);广西卫生厅自筹经费课题(合同号:Z2010314)

摘　　要：目的:研究Bcl-2及Bax在慢性乙型病毒性肝炎(简称慢乙肝)患者肝组织中淋巴细胞的表达与T、B淋巴细胞数量变化的关系。方法:用免疫组织化学的方法,检测57例慢乙肝患者肝脏活检组织和20例肝血管瘤患者外科手术切除标本正常肝组织淋巴细胞中Bcl-2,Bax的分布及数量的变化,并同时进行CD3和CD20免疫组织化学染色,计算T和B淋巴细胞数量变化与表达Bcl-2、Bax的淋巴细胞数量变化的关系。阳性细胞的计数和分析数据均使用病理图像分析仪获得。结果:随着肝炎炎症活动度和纤维化程度的增加,慢乙肝患者肝组织中表达Bcl-2、Bax的淋巴细胞数量增加,同时T、B淋巴细胞数量也增多,均明显多于正常肝组织(P<0.01)。结论:Bcl-2、Bax在慢乙肝患者肝组织淋巴细胞中的表达与T、B淋巴细胞数量的变化有关,并影响慢乙肝患者炎症活动度和纤维化程度的进展。Objective：To investigate Bcl-2 and Bax expression and its relation with the number of T and B lymphocytes in the patients with chronic hepatitis B.Methods-By using immunohistochemical technology,Bcl-2 and Bax positive cells were detected in 57 liver- aspiration-biopsy-specimens from chronic HBV infective patients and 20 normal liver-surgical-specimens from hepatic haemangioma patients.At the same time,CDJ and CD20 immunohistochemical staining was performed,the number of T and B lymphocytes was calculated and Bel-2 and Bax expressions were compared. Positive staining cells were counted and analyzed by pathological image instrument system.Results：With the increasing of hepatitis inflammatory activity and fibrosis,Bcl-2 and Bax expressions were increased,while T,B lymphocyte counting also was increased,the numbers were respectively and significantly more than those of normal liver tissue （P〈0.01）. Conclusion：Bcl-2 and Bax expressions are related to the changes of T,B lymphocytes and affect the inflammatory activity and fibrosis degree of chronic hepatitis B.

关 键 词：慢性乙型病毒性肝炎 细胞调亡 BCL-2 BAX T淋巴细胞 B淋巴细胞 

分 类 号：R36[医药卫生—病理学]