响应面法优化仙鹤草总黄酮的超声提取工艺  

Optimization of Ultrasound Extraction Process of Total Flavonoids from Agrimonia pilosa by Response Surface Methodology

作　　者：蒋志华[1] 俞力超[1] 冯子旺[2] 李峰[1] 徐秀泉[3] 

机构地区：[1]江苏大学附属医院,江苏镇江212001 [2]江苏大学临床医学院,江苏镇江212013 [3]江苏大学药学院,江苏镇江212013

出　　处：《中国实验方剂学杂志》2011年第20期17-20,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：镇江市社会发展项目(SH2010033)

摘　　要：目的:优化仙鹤草总黄酮的最佳超声提取工艺。方法:以总黄酮得率为指标,采用响应面设计方法对影响总黄酮得率的乙醇体积分数、超声温度及料液比进行优化。结果:各因素对总黄酮提取率的影响大小依次为料液比-超声温度-乙醇体积分数。最佳工艺条件为72%乙醇,料液比1∶25,60℃下超声提取20 min。在此最佳条件下,总黄酮得率为9.02 mg.g-1,试验结果与模型预测值相符。结论:利用超声提取仙鹤草总黄酮工艺稳定可靠。Objective： To optimize optimum ultrasound extraction process of total flavonoids from Agrimania pilosa.Method： With yield of total flavonoids as index,ethanol concentration,ultrasound temperature and material-liquid ratio were optimized by response surface methodology（RSM）.Result： Effect factors on extraction rate of total flavonoids with the order of follows： material-liquid ratio,ultrasound temperature,ethanol concentration.The optimal extraction conditions were ethanol concentration was 72%,material-liquid ratio of 1∶25,extraction temperature was 60 ℃,extraction time was 20 min.Under these conditions,yield of total flavonoids reached 9.02 mg·g-1,which was agreed with model prediction.Conclusion： This extraction process of total flavonoids from A.pilosa by ultrasound-assisted was stable and reliable.

关 键 词：仙鹤草 总黄酮 超声提取 响应面设计 

分 类 号：R283.6[医药卫生—中药学]