同步辐射成像应用于大鼠脊髓微循环研究的方法学初探  

A preliminary study of spinal cord microvasculature using synchrotron radiation imaging

作　　者：吴天定[1] 胡建中[1] 曾磊[1] 彭冠云[2] 吕红斌[3] 

机构地区：[1]中南大学湘雅医院脊柱外科,长沙410008 [2]中国科学院上海应用物理研究所,上海201800 [3]中南大学湘雅医院运动医学科运动医学研究中心,长沙410008

出　　处：《核技术》2011年第10期726-730,共5页Nuclear Techniques

基　　金：国家自然科学基金(No.81171698);湖南省自然科学基金(No.09JJ3047);湖南省卫生厅科研基金(No.B2007037)资助

摘　　要：探讨了同步辐射显微断层成像技术(SRμCT)在大鼠脊髓微血管网络研究中的应用前景。正常SD大鼠3只,利用血管造影剂硅酮橡胶经升主动脉行血管造影术,次日取出下胸段脊髓,梯度酒精脱水后,冬绿油保存。标本先利用动物X射线机拍摄普通X片,然后在上海光源X射线成像与生物医学应用光束线站进行扫描和成像,并与原位厚组织切片的光镜摄片进行比较分析。SRμCT能对常规X射线成像无法观察到的大鼠脊髓微循环形态进行有效的成像和图形重构,清晰呈现出大鼠脊髓髓内微血管网络,可分辨的灰质内毛细血管直径最细至7.4μm左右。凭借其超高分辨率,SRμCT在血管相关性脊髓疾病的研究中具有广阔应用前景。Three male SD rats were subjected to angiography via ascending aorta with silicone rubber for investigating rat spinal cord microvasculature images by using synchrotron radiation-based micro-computed tomography. Low thoracic cord was harvested, followed by X-ray radiography, synchrotron radiation micro-imaging and thick histological section in situ. The microvascular images were clearly projected by synchrotron radiation. The rendered 3D images produced more details of the microvascular arrangement in rat spinal cord harmonized with histology. It is of great potential to investigate microvessel-related spinal cord disease with synchrotron radiation-based micro-computed tomography.

关 键 词：同步辐射 断层成像 脊髓 微血管 形态学 

分 类 号：R730.44[医药卫生—肿瘤]