混合菌剂固态发酵棉粕脱毒条件的优化  

Optimization of Detoxification Conditions of Cottonseed Cake by Solid-state Fermentation with Mixed Culture

作　　者：金红春[1] 兰时乐[2] 王红权[1] 毛小伟[1] 黄云[1] 肖调义[1] 

机构地区：[1]湖南农业大学动物科技学院,长沙410128 [2]湖南农业大学生物科学技术学院,长沙410128

出　　处：《中国农学通报》2011年第24期50-56,共7页Chinese Agricultural Science Bulletin

基　　金：湖南省科技厅重点项目"主要名特水产健康养殖关键技术研究与示范"(2008NK2004)

摘　　要：为找到一种脱毒较为彻底且稳定的棉粕脱毒方法,以复合芽孢杆菌为发酵剂,通过添加由木聚糖酶、纤维素酶和甘露聚糖酶组成的复合酶研究其协同微生物固体发酵棉粕脱毒的发酵过程参数。采用单因素试验确定了复合酶协同微生物发酵棉粕脱毒培养基及脱毒条件,在此试验结果的基础上,采用正交试验对发酵脱毒的主要因素进行研究,用SPSS17.0软件分析试验数据。确定了酶法协同微生物固体发酵脱毒工艺条件为:棉粕:麦麸=90:10,(NH4)2SO41%,复合酶40U/g底物,发酵温度40℃,发酵初始pH6.0,含水量50%,接种量6%(V/W),装量为70g/250mL三角瓶,发酵时间为6天。在此条件下,游离棉酚的去除率达96.52%。In order to obtain an effective and stable method,the collaborative solid-state fermentation for detoxification of cottonseed meal were processed by adding xylanase,cellulase and mannase,and the parameters of fermentation were optimized in the compound bacillus agent fermenting.The fermentation medium and detoxification conditions were optimized by single-factor test.Then,the optimum composition of the crucial factors of fermentation detoxification was optimized by orthogonal test.The test date was farther analyzed with SPSS 17.0 software.The appropriate conditions of solid fermentation for detoxification of cottonseed meal with the collaborative enzymes were as follows：cottonseed meal：wheat bran=90：10,（NH 4） 2 SO 4 1%,complex enzyme 40 U/g substrate,fermentation temperature 40℃,the initial pH 6.0,moisture content 50%,the vaccination quantity 6%（V/W）,70 g solid medium in 250 mL triangular flask,fermentation time 6 days.Under the technique conditions,the removal rate of free gossypol reached 96.52%.

关 键 词：固态发酵 游离棉酚 棉粕 脱毒 正交设计 

分 类 号：Q81[生物学—生物工程]