烟蚜2种微卫星标记银染方法的比较  

Comparison of Two Silver Staining Methods for Polyaerylamide Gels Electrophoresis by Using SSR Markers on Myzus persicae(Sulzer)

作　　者：吕召云[1,2] 杨茂发[1,2] 商胜华[3] 

机构地区：[1]贵州大学昆虫研究所,贵阳550025 [2]贵州山地农业病虫害重点实验室,贵阳550025 [3]贵州省烟草科学研究所,贵阳550025

出　　处：《中国农学通报》2011年第24期273-276,共4页Chinese Agricultural Science Bulletin

基　　金：中国烟草总公司贵州省公司科技项目"贵州省烟草有害生物调查研究"(201022)

摘　　要：实验比较了烟蚜SSR-PCR产物聚丙烯酰胺凝胶电泳2种改良银染方法,目的是为初用者在选择银染方法时提供参考。用非变性聚丙烯酰胺凝胶电泳检测烟蚜SSR-PCR的产物,结果表明银染方法2灵敏度高、简便省时,更适于对烟蚜遗传多样性的研究。Two ways of DNA improved silver staining methods for polyacrylamide gels electrophoresis on Myzus persicae（Sulzer） were compared in this article for first detection.The detected results with the technique of SSR markers by non-denaturant polyacrylmaaide gel eletrophoresis indicated that the second method was characterized as more sensitive and simple steps when compared with the first method.And for saving time and work the second method was more appropriate to the genetic diversity of Myzus persicae（Sulzer）.

关 键 词：烟蚜 银染 非变性聚丙烯酰胺凝胶 SSR-PCR 

分 类 号：Q963[生物学—昆虫学]