Phyllobacterium myrsinacearum F2的分离和对吲哚醌的降解  

Isolation of Phyllobacterium myrsinacearum F2 and degradation of isatin

作　　者：王雁萍[1] 董湘熔[1] 李月艳[1] 谈重芳[1] 李宗伟[1] 常胜和[1] 秦广雍[1] 

机构地区：[1]河南省离子束生物工程重点实验室,河南郑州450052

出　　处：《工业微生物》2011年第5期22-26,共5页Industrial Microbiology

基　　金：农业部农业公益性行业科研专项经费项目(200803034);河南省教育厅自然科学研究计划项目(2011A180030);河南省重点科技攻关计划项目(112102310069)

摘　　要：从受到污染的吲哚醌溶液中分离到菌株F2,该菌株能够快速高效地降解吲哚醌。根据其形态特征、生理生化指标及16S rRNA基因序列分析结果,该菌株被鉴定为紫金牛叶杆菌(Phyllobacterium myrsinacearum)。F2菌株为好氧菌,在10℃～40℃的温度条件下对吲哚醌均有降解作用,适宜温度范围为25℃～35℃;在28℃条件下,F2菌株能够有效降解50～500 mg/L的吲哚醌。该菌株在漆酶脱色靛蓝后的印染废水处理中将能起到重作用。A highly effective isatin-degrading strain F2 was isolated from contaminated isatin solution. This strain was identified as Phyllobacterium myrsinacearum based on its morphological, physiological and biochemical properties, combined with 16S rRNA gene sequence analysis. F2 strain belongs to aerobic bacteria. It could degrade isatin under 10 --40 ℃, and the proper temperature was in the range of 25 ℃ to 35 ℃. It could degrade 50--500 mg/L isatin at 28 ℃. The results showed that F2 strain could play an important role in the treatment of indigo wastewater after pretreatment with laccase.

关 键 词：生物降解 吲哚醌 紫金牛叶杆菌 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]