荧光显微成像技术应用于生物样品中氧氟沙星的检测  

Determination of the Ofloxacin in the Biologic Samples by Fluorescence Microscopic Imaging Technique

作　　者：刘颖[1] 余燕敏[1] 李慧[1] 李金淑[1] 

机构地区：[1]中央民族大学生命与环境科学学院,北京100081

出　　处：《光谱学与光谱分析》2011年第11期2981-2986,共6页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(20767004;21177163);国家民委科研项目(09ZY011);中央民族大学"985工程"项目(MUC98504-14;MUC98507-08);中央民族大学"211工程"项目(MUC021211306);中央民族大学2009年度研究生"自主科研项目"(2009kyqnyjs23)资助;高等学校学科创新引智计划(B08044);中央高校基本科研业务费专项资金(0910KYZY45)

摘　　要：应用毛细流自组装成环荧光显微成像技术,建立了溴化十六烷基三甲胺(Cetyltrimethylammoniumbromide,CTMAB)-Al 3+-氧氟沙星(Ofloxacin,OFLX)三元荧光体系(CTMAB-Al 3+-OFLX)测定氧氟沙星的方法,并实测鸡灌喂氧氟沙星药片后血液中药物浓度、人体尿液中氧氟沙星浓度及蜂蜜、氧氟沙星片剂、滴眼液中氧氟沙星的含量,均得到满意结果。在pH9.50NH3-NH4Cl缓冲介质和聚乙烯醇-124存在下,溴化十六烷基三甲胺-Al 3+-氧氟沙星三元配合物在疏水性玻璃表面上受热挥发形成的自组装环直径为1.63mm,环线宽为50μm。当点样体积为0.20μL,氧氟沙星在3.30×10-13～1.65×10-12 mol.ring-1(0.60～2.98mg.L-1)范围内,荧光强度与药物浓度有良好的线性关系(r=0.999 2),检测限为4.10×10-15 mol.ring-1(7.41μg.L-1)。此法用于鸡灌胃氧氟沙星2h后血药中药物浓度的测定,回收率为96.4%～101.2%;检测了三名健康志愿者服用氧氟沙星片剂后尿液中氧氟沙星的浓度,发现服药后3h尿液中药物浓度最高,回收率在98.2%～106.1%。应用于三种蜂蜜中氧氟沙星残留量的测定,回收率为98.2%～106.1%,相对标准偏差小于2.3%;检测了氧氟沙星片剂、滴眼液中有效成分的含量,与标示量相近,回收率分别为93.5%～101.5%和95.8%～104.2%,RSD为3.5%和3.6%。The method of CTMAB-Al3＋-OFLX ternary complex fluorescence microscopic imaging technique was established for the determination of ofloxacin based on the capillary effect of solvent on solid supports,and the concentration in the serum after the chicken was burdened with ofloxacin tablet,the concentration in the human urines and the percentage composition in the honeies,ofloxacin tablets and eye-drops were measured with satisfaction,respectively.In the presence of pH 9.50 NH3-NH4Cl buffer solution and PVA-124,CTMAB-Al3＋-OFLX ternary complex can form a self-ordered ring on the hydrophobic supports with the diameter of 1.63 mm and its ring belt width of 50 μm.When a 0.20 μL droplet was spotted,the fluorescence intensity of the ring had a favorable linear relation（r=0.999 2） with the drug concentration in the range of 3.30×10-13~1.65×10-12 mol·ring-1（0.60~2.98 mg·L-1） and the limit of detection can reach 4.10×10-15 mol·ring-1（7.41 μg·L-1） with three times of signal to noise ratio.This method has been applied to the average concentration of ofloxacin in the chicken serum with the recovery of 96.4%~101.2% after two hours of being burdened with ofloxacin tablet.Then the technique was applied to the determination of ofloxacin in the three healthy volunteer＇s urines after oral administration with recovery of 98.2%~106.1%.It was found that the concentrations of ofloxacin in urines were the highest after three hours of taking medicine;the result was similar to reports in the literature.The residues of ofloxacin in three different honey samples were satisfactorily determined with the recoveries of 98.2%~106.1%,and RSD was less than 2.3%.The contents of active constituent in tablet samples and eye-drops sample were determined with recoveries of 93.5%~101.5% and 95.8%~104.2%,and RSD was 3.5% and 3.6%,respectively,which were similar to marked values.

关 键 词：自组装成环荧光显微成像技术 氧氟沙星 鸡血清 人体尿液 蜂蜜 氧氟沙星片剂 

分 类 号：O482.3[理学—固体物理]