TDGF-1基因下调对人鼻咽癌细胞生长迁移侵袭的影响  

Impacts of TDGF-1 down-regulation by small interfering on invasion and migration of human nasopharyngeal cancer cells

作　　者：沈荣[1] 范钰[2] 龚丹丹[2] 

机构地区：[1]江苏大学附属人民医院耳鼻喉科,江苏镇江212002 [2]江苏大学附属人民医院肿瘤研究所

出　　处：《中国肿瘤外科杂志》2011年第5期290-293,共4页Chinese Journal of Surgical Oncology

基　　金：江苏省自然科学基金资助项目(BS2009205)

摘　　要：目的探讨TDGF-1基因小干扰RNA(small interfering RNA,siRNA)对人鼻咽癌细胞迁移侵袭的影响。方法根据TDGF-1基因序列特点设计了siRNA,转染人鼻咽癌CNE-2细胞,分别采用实时定量PCR和蛋白质印迹法检测TDGF-1基因的mRNA和蛋白水平,以软琼脂集落形成试验观察癌细胞锚着不依赖性增殖能力,以划痕试验方法评测癌细胞的迁移能力;用Boyden模型观察癌细胞侵袭能力。结果 TDGF-1基因siRNA转染组TDGF-1 mRNA和蛋白水平明显下调,且呈浓度和时间依赖性(均P<0.001)。体外试验发现,TDGF-1 siRNA转染可有效抑制鼻咽癌细胞集落生长、侵袭和迁移能力,且与浓度相关(均P<0.005)。结论 TDGF-1在鼻咽癌细胞生长、迁移、侵袭中起着重要的作用;采用TDGF-1 siRNA转染可抑制鼻咽癌细胞侵袭。Objective To study the effects of TDGF-1 small interfering RNA （siRNA） on invasion and migration of human nasopharyngeal cancer cells. Methods After human nasopharyngeal cancer cell line CNE-2 was transfected by TDGF-1 siRNA, the mRNA and protein expression level of TDGF-1 were determined by real time-PCR and Western blot assay, respectively. The anchorage-independent growth was examined by clone formation in soft agar, and the ability of migration and invasion was evaluated by wound-healing assay and boyden chamber model, respectively. Results The siRNA could down-regulate the level of mRNA and protein of TDGF-1 in a dose- and time- dependent manner. Suppression of TDGF-1 expression could inhibit anchorage-in- dependent growth in a dose-dependent manner, and invasion, and migration ability of nasopharyngeal cancer cell. Conclusions TDGF-1 gene might play an important role in the development of human nasopharyngeal cancer. Down-regulated TDGF-1 by siRNA could inhibit growth, invasion and migration of human nasopharyngeal cancer cells.

关 键 词：鼻咽癌 畸胎瘤衍生生长因子-1基因 癌细胞迁移 癌细胞侵袭 癌细胞生长 小干扰RNA 

分 类 号：R739.63[医药卫生—肿瘤]