死亡结构域相关蛋白和E2启动子结合因子在儿童急性白血病中的表达及其临床意义  

The expression and clinical implications of Daxx and E2F1 in childhood acute leukemia

作　　者：张宝玺[1] 王素明[2] 吴晓莉[1] 赵晓庆[1] 张爱辉[1] 封雪枫[1] 

机构地区：[1]河北医科大学第二医院儿科,石家庄050000 [2]石家庄医学高等专科学校新华校区,石家庄050071

出　　处：《中国小儿血液与肿瘤杂志》2011年第5期217-220,共4页Journal of China Pediatric Blood and Cancer

摘　　要：目的探讨死亡结构域相关蛋白(Daxx)和E2启动子结合因子-1(E2F1)在急性白血病(AL)患儿骨髓细胞中的表达及其临床意义。方法采用免疫组织化学链霉菌抗生物素蛋白-过氧化物酶连结(streptavidin peroxidase,SP)法,分别检测40例AL患儿和20例非恶性血液病儿童Daxx和E2F1的表达情况。结果 (1)AL组患儿骨髓细胞Daxx和E2F1阳性表达率分别为40%和57.5%,均显著高于对照组的10%(P<0.05)和20%(P<0.01);Daxx表达水平在急性髓细胞白血病组(58.8%)明显高于急性淋巴细胞白血病组(26.1%,P<0.05);E2F1在两组患儿中的阳性表达率差异无显著性(P>0.05)。(2)Daxx表达阴性者的完全缓解率高于阳性者(P<0.05),但E2F1表达阳性者与阴性者的完全缓解率相比差异无显著性(P>0.05)。(3)AL患儿Daxx与E2F1的表达水平有相关关系(P<0.05)。结论 Daxx和E2F1在儿童急性白血病中高水平表达,在儿童急性白血病的发生发展过程中可能存在某种协同作用,可能与儿童AL的疗效及预后相关。ObjectiveTo explore the expression levels of death domain associate protein（Daxx） and E2 promoter binding factor-1（E2F1）in patients with childhood acute leukemia（AL）and evaluate their clinical significance.MethodsThe expression of Daxx and E2F1 in 40 AL patients and 20 nonmalignant hematologic patients were measured by immunohistochemical streptavidin-perosidase（IHC,SP）.ResultsThe expression rates of Daxx and E2F1 in AL patients（40% and 57.5%）were higher than those in control group（10.0% and 20.0%）（P〈0.05 and P〈0.01）.The expression rate of Daxx in AML patients（58.8%）was higher than that in ALL cases（26.1%）（P〈0.05）.The expression rate of E2F1 in AML and ALL patients had no significant difference（P〈0.05）.The complete remission（CR） rate of patients with negative expression of Daxx was higher than that in positive group（P〈0.05）.There was no significant difference of CR rate between the positive and negative expression groups of E2F1（P〈0.05）.It showed correlation between Daxx and E2F1 expression in newly dianosed AL（P〈0.05）.ConclusionsThere was a high expression of Daxx and E2F1 in AL patients,which may be associated with the leukemogenesis of childhood AL and related to the treatment outcome and prognosis.

关 键 词：死亡结构域相关蛋白 E2启动子结合因子-1 儿童 急性白血病 免疫组织化学 

分 类 号：R733.71[医药卫生—肿瘤]