马克斯克鲁维酵母对柑橘采后绿霉病的抑制效果  

Combination of Kluyveromyces marxianus and ammonium molybdate for control of green mold caused by Penicillium digitatum on citrus fruits

作　　者：耿鹏[1] 陈少华[1] 胡美英[1] 瞿菲[1] 张彦博[1] 安国栋[1] 

机构地区：[1]华南农业大学天然农药与化学生物学教育部重点实验室,广州510642

出　　处：《华中农业大学学报》2011年第6期712-716,共5页Journal of Huazhong Agricultural University

基　　金：国家自然科学基金项目(30871660);广东省科技计划项目(2008B020900007)

摘　　要：通过活体(in vivo)和离体(in vitro)试验,研究拮抗酵母菌株Kluyveromyces marxianus与不同浓度钼酸铵混合使用对柑橘采后绿霉病(Penicillium digitatum)的防治效果,以及钼酸铵对K.marxianus菌株生长和繁殖的影响。结果表明:不同浓度的钼酸铵对K.marxianus的生长和繁殖没有不利影响;钼酸铵对酵母菌在柑橘果实伤口处的生长和繁殖有刺激作用;当K.marxianus与浓度为1mmol/L钼酸铵混合使用时对控制柑橘果实绿霉病的效果最好,3和6d的发病率分别为28.33%和60.00%,显著低于对照处理的93.33%和100%的发病率,表明K.marxianus与钼酸铵混合使用在控制柑橘绿霉病方面具有潜在的应用价值。With the methods of in vivo and in vitro,the potential enhancement of Kluyveromyces marxianus by ammonium molybdate（NH4Mo） to control green mold caused by Penicillium digitatum on citrus fruit was investigated.Meanwhile,the effect of NH4Mo on growth and proliferation of K.marxianus was tested.The results showed that NH4Mo at different concentration did not inhibit the growth and proliferation of K.marxianus in culture medium or wounds of citrus fruits.The combination of K.marxianus and NH4Mo had suppressive effect on green mold caused by P.digitatum.Treatment of citrus fruits with K.marxianus plus NH4Mo（1 mmol/L） resulted in a consistent biocontrol activity,the disease incidence after 3 and 6 days were 28.33% and 60.00%,respectively.While the control treatment resulted in a disease incidence as high as 93.33% and 100%,suggesting that the combination of K.marxianus with NH4Mo may have great potential against green mold of citrus fruits.

关 键 词：拮抗酵母菌 绿霉病 生防效果 采后病害 钼酸铵 柑橘 

分 类 号：S436.33[农业科学—农业昆虫与害虫防治] S482.2[农业科学—植物保护]