食管癌TE-1细胞生物学性状的相关性研究  

Study on the biological characteristics of the esophageal cancer cells TE-1

作　　者：李步强[1] 许健[1] 李奇志[1] 李晓义[1] 谷锋泽[1] 慕晓玲[1] 

机构地区：[1]新疆石河子大学医学院组胚教研室,832002

出　　处：《中国现代医药杂志》2011年第10期33-36,共4页Modern Medicine Journal of China

摘　　要：目的研究食管癌TE-1细胞生物学性状。方法①将购自上海生命科学院的食管癌TE-1细胞株进行HE染色;②用MTT法绘制细胞生长曲线;③免疫组织化学检测细胞特异性免疫标志;④流式细胞仪分析细胞周期。结果①用DMEM高糖培养基培养,生长良好。HE染色下,细胞大致分为3种:一种细胞呈椭圆形,核大,胞浆少,一种细胞呈三角形或多角形,大小,形态不等,一种细胞有双核或者多核,核大深染,染色质丰富,核分裂相多见;②细胞接种培养后其生长曲线呈近似S形。免疫组织化学检测3种抗体均表达阳性,其中CK7阳性表达定位于胞浆,Ki-67和p53定位于胞核;③TE-1细胞周期:半数细胞处于增生期,半数处于静止期。结论食管癌TE-1细胞符合食管鳞癌的特征。Objective To study the biological characteristics of esophageal cancer cells. Methods ①The morphological characteristicas was observed in HE staining.②The cell grouth curve was analysed by MTT method. ③The immunocytochemical method was employed to detect the specific cell phenotype. ④The cell cycle was analysed by FCM. Results The cells grew well in the high glucose DMEM. The cells can be divided into three of kinds in HE staining,the first cells were ovalshaped, nucleus large and small cytoplasm, the second were triangular or polygonal, and the third had dual-nucleus or multi- nucleus and nucleus mitotic figures were common. After the cells were planted, the cells in the first-second day were in the in- cubation period, the third-fifth day was in proliferative index period and in the seventh day, the cells were into the plateau growth period. The CK7 expression was positive in the cytoplasm. Ki-67 positive expression were in the nucleus. P53 positive expression were in the nucleus too.Half of the cells were in the proliferative phase of cell cycle and half in the quiescent phase. Conclusion TE-1 cells line have the biological characteristics of esophageal squamous cell carcinoma.

关 键 词：食管癌 TE-1细胞株 生物学特性 

分 类 号：R735.1[医药卫生—肿瘤]