沙利度胺对小鼠食管癌血管形成的影响  

The effect of thalidomide on esophageal cancer angiogenesis

作　　者：王喜安[1] 沈若宇[1] 穆红艳[1] 陈云[2] 

机构地区：[1]解放军第四一一医院特诊科,上海200081 [2]解放军第四一一医院病理科,上海200081

出　　处：《海军医学杂志》2011年第5期305-307,共3页Journal of Navy Medicine

摘　　要：目的研究沙利度胺对食管癌血管形成的影响,并初步探讨其抑瘤机制。方法建立裸鼠食管癌原位移植瘤模型,将30只小鼠随机分为3组,每组10只。种植后3 d开始,分别腹腔注射生理盐水(0.5 ml/d,对照组)、环磷酰胺[30 mg/(kg.d),环磷酰胺组]、沙利度胺[50 mg/(kg.d),沙利度胺组],1次/d,持续到接种后第14天。采用Envision System免疫组织化学方法,检测肿瘤组织微血管密度(MVD)、增殖细胞核抗原(PCNA)和血管内皮细胞生长因子(VEGF)。结果对照组、环磷酰胺组、沙利度胺组MVD为(12.8±4.2)(、11.5±2.8)(、2.3±1.1),PCNA指数为(61.1±6.1)%(、66.2±4.2)%、(53.6±2.9)%,VEGF阳性率为(9.5±3.3)%(、5.1±2.9)%(、0.8±0.2)%。沙利度胺组MVD、VEGF阳性率均低于对照组,差异有统计学意义(P<0.05)。环磷酰胺组、沙利度胺组抑瘤率分别为79.8%5、3.1%。结论沙利度胺通过抑制小鼠食管癌的血管生成,抑制肿瘤生长,联合使用其他药物,将在实体瘤的治疗中具有良好的应用前景。Objective Study the vascular suppressive effect of thalidomide on esophageal cancer and the suppressive mechanism. methods The esophageal cancer TE1 cell strain was injected subcutaneously in general Kun-Ming mice to make animal experimental model.There were four groups in the model,the control group（saline solution 0.5ml,ip）,the Cy group（cyclophosphane 30 mg/（kg·d）,ip）,thalidomide groups（50 mg/（kg·d）,ip）.The MVD、PCNA and VEGF were observed using Envision System immunohistochemistry.results In thalidomide groups the MVD were 2.3±1.1,the positive rate of VEGF were（0.76±0.2）%.Both the MVD and the VEGF were significantly lower than the control group（P0.05）.The suppressive rate of thalidomide groups were 53.1%. conclusion Thalidomide can inhibit the growth of mice esophageal cancer TE1 through its antiangiogenic function.So it will have a very strong potential perspective in the future.

关 键 词：沙利度胺 食管癌 新生血管化 

分 类 号：R733.3[医药卫生—肿瘤]