白腐菌预处理木屑产漆酶条件的优化及其糖化评价  

Optimization of Laccase-producing Conditions of Wood Chips Pretreated by White-rot fungi and Evaluation of Its Saccharification

作　　者：罗少华[1] 程金花[1] 龚大春[1] 

机构地区：[1]三峡大学化学与生命科学学院,湖北宜昌443002

出　　处：《酿酒科技》2011年第10期17-20,共4页Liquor-Making Science & Technology

基　　金：湖北省教育厅重点项目(CXY2009B008);国家自然基金项目(21076114)

摘　　要：对白腐菌固态发酵预处理木屑的工艺条件进行了优化,并对经过白腐菌预处理木屑的糖化效果进行了评价。结果表明,白腐菌固态发酵产漆酶的工艺条件为:1 kg木屑中需添加麦麸120 g,氮酒石酸铵60 g,料水比0.28,微量元素的用量为7 mL/L,培养时间为3 d,白腐菌产漆酶的酶活最高。在最佳工艺条件下,经白腐菌生物预处理的木屑的糖化率可以提高1倍,生物预处理木屑具有一定的应用前景。The technical conditions of wood chips pretreated by white-rot fungi solid fermentation had been optimized as follows： 120g wheat bran added in per kg wood chips as carbon source and 60 g tartaric acid ammonium added as nitrogen source,the ratio of raw materials and water was 0.28,the use level of trace elements was 7 ml/L,and culture time was 3 d.Besides,the saccharifying effects of wood chips were evaluated.Under the above conditions,the activity of the produced laccase was the highest and the saccharifying rate of wood chips increased by one time.Accordingly,wood chips pretreated by biological measures had bright application prospects.

关 键 词：微生物 白腐菌 漆酶 优化 预处理 糖化 

分 类 号：TQ925[轻工技术与工程—发酵工程]