氧化亚铜和氢氧化铜对苦草抗氧化酶活性和叶绿素含量的影响  

Effects of Cuprous Oxide(Cu_2O) and Cupic Hydroxide [Cu(OH)_2] on Activity of Antioxidase and Content of Chlorophyll in Vallisneria natans

作　　者：胡芳[1] 浦海清[1] 陈良燕[1] 程树培[1] 

机构地区：[1]污染控制与资源化研究国家重点实验室/南京大学环境学院,江苏南京210093

出　　处：《生态与农村环境学报》2011年第5期72-78,共7页Journal of Ecology and Rural Environment

基　　金：国家水体污染控制与治理科技重大专项(2008ZX07316)

摘　　要：研究了不同浓度氧化亚铜和氢氧化铜可湿性粉剂染毒对苦草叶片抗氧化酶〔超氧化物歧化酶(SOD)、抗坏血酸过氧化物酶(APX)和过氧化物酶(POD)〕比活力、丙二醛(MDA)含量和叶绿素含量的影响。结果表明,以0.15～1.5 mg.L-1氧化亚铜可湿性粉剂对苦草染毒7 d,其叶片SOD、APX和POD活性明显升高,1.5 mg.L-1处理组3种抗氧化酶比活力分别比助剂对照组升高49.0%、77.0%和39.0%,MDA含量极显著上升46.9%,总叶绿素含量显著下降42.6%。染毒14 d后3种抗氧化酶比活力均有不同程度回落,MDA含量恢复至正常水平,总叶绿素含量也有所回升。以0.1～1.0 mg.L-1氢氧化铜对苦草染毒7 d,1.0 mg.L-1处理组3种抗氧化酶比活力和MDA含量也呈显著上升趋势,总叶绿素含量显著下降。染毒14 d时3种抗氧化酶比活力和MDA含量恢复到正常水平,总叶绿素含量回升。上述结果显示,在氧化亚铜和氢氧化铜引起的铜胁迫早期,苦草叶片抗氧化酶因过氧化应激响应而上升,膜脂出现一定程度过氧化损伤,叶绿素含量下降,但随着暴露时间的延长,苦草对铜胁迫产生适应性,过氧化损伤得以缓解,叶绿素含量回升。Effects of two wettable copper powders,cuprous oxide（Cu2O） and cupic hydroxide [Cu（OH）2）],varying in concentration on activity of antioxidases [superoxide dismutase（SOD）,ascorbate peroxidase（APX）,and peroxidase（POD）],content of malondialdehyde（MDA） and content of chlorophyll in leaves of Vallisneria natans were studied.Results show that exposure to Cu2O W.P.（0.15-1.5 mg·L-1） for 7 days significantly increased specific activities of superoxide dismutase（SOD）,ascorbate peroxidase（APX） and peroxidase（POD） in the leaves of the plant.In the treatment,highest in concentration of the pollutant（1.5 mg·L-1）,specific activities of the three anti-oxidative enzymes increased by 49.0%,77.0% and 39.0%,respectively,and content of MDA dramatically increased by 46.9%,but content of total chlorophyll decreased by 42.6%.After the plant was exposed to the toxicant for 14 days,the specific activities of the three anti-oxidative enzymes in the plant receded to a varying extent,the content of MDA returned to its normal level,and the content of chlorophyll somewhat recovered.When exposed to Cu（OH）2 W.P.（0.1-1.0 mg·L-1） for 7 days,the plant in the treatment highest in concentration（1.0 mg·L-1） of the toxicant showed a similar trend in specific activities of anti-oxidative enzymes,MDA level and chlorophyll content.And when the exposure lasted for 14 days,the activities of anti-oxidative enzymes and the MDA level fell back to their normal ranges,and the content of total chlorophyll also went up.The above-listed findings indicate that at the early stage of the cupper stress caused by cuprous oxide and cupic hydroxide,activities of the anti-oxidative enzymes in Vallisneria natans ascend in response to oxidative stress,causing certain oxidative damage to membrane lipid and decline of chlorophyll content.But with duration of the exposure extending,the plant gradually gets adapted to the stress,with oxidative damage mitigated and chlorophyll content recovered.

关 键 词：氧化亚铜 氢氧化铜 苦草 抗氧化酶 丙二醛(MDA) 叶绿素 

分 类 号：X171.5[环境科学与工程—环境科学]