人喉癌组织及喉癌细胞系肿瘤干细胞表面标记的差异  

Differential expression of cancer stem cell surface markers in human laryngocarcinoma and the cell lines

作　　者：洪锐[1] 钟琦[2] 宋舸[3] 黄志刚[2] 

机构地区：[1]北京市石景山医院耳鼻咽喉科,北京100043 [2]首都医科大学附属北京同仁医院耳鼻咽喉头颈外科耳鼻咽喉头颈科学教育部重点实验室(首都医科大学),北京100730 [3]中国医学科学院医学实验动物研究所,北京100021

出　　处：《中国耳鼻咽喉头颈外科》2011年第9期461-464,共4页Chinese Archives of Otolaryngology-Head and Neck Surgery

基　　金：高等学校博士学科点专项科研基金(20091107110007)

摘　　要：目的比较细胞膜表面蛋白CD44、ABCG2(ATP-binding cassette superfamily G2,ABCG2)和CD133在人喉鳞状细胞癌(简称喉癌)组织及喉癌Hep-2、LSC-1细胞系中的表达及其差异性,从而探讨在喉癌肿瘤干细胞筛选中的价值。方法以人喉癌组织及喉癌细胞系Hep-2、LSC-1为研究对象,应用流式细胞仪检测CD44、ABCG2和CD133在人喉癌组织及喉癌细胞系中的表达情况。结果 CD44在喉癌组织中表达个体差异性较大,在喉癌细胞系中稳定高表达。喉癌组织与喉癌细胞系中表达量不同,ABCG2在喉癌组织中阳性表达、Hep-2中呈弱阳性表达、LSC-1中呈高表达,在喉癌组织与喉癌细胞系Hep-2中表达差异无统计学意义。CD133在喉癌组织中阳性表达,在喉癌细胞系中呈弱阳性表达,且喉癌组织与喉癌细胞系表达差异无统计学意义。结论 CD44、ABCG2及CD133在喉癌组织与喉癌细胞系中的表达存在明显差异。OBJECTIVE To compare the expression of CD44、ABCG2 and CD133 in laryngocarcinoma tissue from patients and laryngocarcinoma cell lines Hep2、LSC-1,to analyze the variability of expression among them,thus to make a approach to the value of the above-mentioned antibodies on screening cancer stem cells in laryngocarcinoma.METHODS By using laryngocarcinoma tissue from patients and laryngocarcinoma cell lines Hep2、LSC-1 as object,then labeling cells with CD44、ABCG2 and CD133,we analyzed the expression of the above-mentioned antibodies in laryngocarcinoma cells on FACSAriaTM Cell Sorter.RESULTS Distinct populations of CD44 were identified in solid tumors of laryngocarcinoma patients with strong individual deviations,constitutively expressed on the surface of laryngocarcinoma cell lines analyzed.There were significant difference between the CD44 expression in laryngocarcinoma tissue and that in the cancer cell lines.ABCG2 expressed positively in laryngocarcinoma tissue,dim positively in Hep-2,but intensive positively expressed almost in all LSC-1 cells.And there was no significant difference between the expression of ABCG2 in laryngocarcinoma tissue and that in Hep-2.CD133 expressed positively in laryngocarcinoma tissue,dim positively in both cancer cell lines.and there was no significant difference for the expression of CD133 between laryngocarcinoma tissue and the cancer cell lines.CONCLUSION The expression of CD44、ABCG2 and CD133 makes a conspicuous difference between laryngocarcinoma tissue and laryngocarcinoma lines.

关 键 词：癌 鳞状细胞 肿瘤干细胞 抗原 表面 细胞系 肿瘤 流式细胞术 

分 类 号：R739.65[医药卫生—肿瘤]