三氧化二砷对淋巴瘤Raji细胞的抑制效应研究  

DEPRESSIVE EFFECT OF ARSENIC TRIOXIDE ON RAJI LYMPHOMA CELLS

作　　者：曲凡[1] 赵春华[1] 刁玉巧[1] 朱秀丽[1] 陈健[1] 李梅[1] 

机构地区：[1]河北医科大学第四医院儿科,河北石家庄050011

出　　处：《河北医科大学学报》2011年第9期1024-1027,共4页Journal of Hebei Medical University

基　　金：河北省卫生厅医学科学研究重点课题计划(20090493)

摘　　要：目的研究三氧化二砷(As_2O_3)对恶性淋巴瘤细胞的的影响。方法体外培养人Burkitts淋巴瘤细胞株Raji细胞,设对照组和用不同浓度As_2O_3作用不同时间处理Raji细胞的实验组。应用四甲基偶氮唑盐(3-(4,5-dimethyl-2-thiazoly)-2,5-diphenyl tetrazolium dromide,MTT)比色法检测细胞生长情况;流式细胞术检测细胞凋亡并分析As_2O_3对细胞周期的影响。结果①实验组MTT比色实验结果表明,As_2O_3各浓度组作用于Raji细胞24、48、72h后抑制率为12.15%～92.17%(P<0.05);②流式细胞术检测细胞凋亡结果显示,实验组As_2O_3各浓度组作用于Raji细胞48h后出现明显的凋亡峰,对照组未见凋亡峰或仅有低平的凋亡峰;③流式细胞术细胞周期分析结果显示,实验组As_2O_3各浓度组作用于Raji细胞48h后,As_2O_3可阻滞Raji细胞于G_0/G_1期,且随着As_2O_3浓度的增加,阻滞作用增强,呈一定剂量依赖关系。结论①As_2O_3体外对Raji淋巴瘤细胞生长具有明显的抑制作用;②As_2O_3能诱导Raji细胞凋亡,且使G_2/G_1期细胞比例增加,同时S期及G_2/M期细胞比例减少。Objective To investigate the effect of arsenic trioxide （As2O3 ）on malignant lymphoma cells. Methods Human Burkitt＇s Raft lymphoma cells were cultivated in vitro. Two groups were designed as control group and experiment group, the latter was exposed to various concentrations of As2O3 for different time. The growth of cells was tested by methyl thiazolyl tetrazolium （MTT）colorimetry. Apoptosis and cycle distribution of cells were detected by flow cytometry （FCM）. Results ①MTT test showed that the inhibition rate of Raji after treated with various concentrations of As2O3 for 24,48,72 hours were among 12.15% -92.17% （P 〈 0.05）. ②FCM showed that obvious apoptosis peak emerged after Raji cells treated with As2O3 for 48 hours in the experiment group,but there was no or only a low apoptosis peak in the control group. ③FCM showed that after Raft cells were treated with various concentrations of As2O3 for 48 hours in experiment groups,more cells were arrested in G0/G1 phase. The G0/G1 arrest effect increased with the increasing of As2O3 concentration in a dose - dependent manner. Conclusion ①The Raji cells was depressed effectively by As2O3 in vitro. ②@As2O3 can induce the apoptosis in Raji cells, increase the Raji cells in G0/G1 phase, and decrease the cells in S and G2/M phase.

关 键 词：淋巴瘤 三氧化二砷 细胞 

分 类 号：R733.4[医药卫生—肿瘤]