致炎剂诱发清醒大鼠反复发作头痛及对PAG区c-Fos表达的影响  

INFLAMMATORY SOUP INDUCES RECURRENT HEADACHE IN AWAKE BEHAVING RATS AND CONTRIBUTES TO THE EXPRESSION OF C-FOS IN PAG AREA

作　　者：薛刘军[1] 周志奎[1] 叶青[1] 武文卉[1] 刘欣[1] 刘沙[1] 万琪[1] 

机构地区：[1]南京医科大学第一附属医院神经内科,南京210029

出　　处：《中国疼痛医学杂志》2011年第10期616-619,共4页Chinese Journal of Pain Medicine

基　　金：国家自然科学基金(81070896)资助课题

摘　　要：目的:采用复方致炎剂(IS)定期刺激大鼠硬脑膜,建立一种清醒状态下大鼠反复发作性头痛模型。方法:在雄性SD大鼠硬脑膜上埋置PE-10管,连续6天给予IS或等量生理盐水。采用von Frey毛测试大鼠眶周的压力疼痛阈值,将左侧硬脑膜行甲苯胺蓝染色观察肥大细胞脱颗粒比率,通过免疫组化染色技术对六次NS及IS和一次IS给药组大鼠的中脑导水管灰质(PAG)区域c-Fos阳性细胞进行计数观察。结果:随着IS刺激次数的增多大鼠的眶周压力疼痛阈值下降;肥大细胞脱颗粒比率较NS组明显增加(P<0.01);c-Fos阳性细胞数IS两组较NS组有显著差异(P<0.01),IS两组之间无差异(P>0.05)。结论:反复给予复方致炎剂可以成功诱导大鼠反复发作性头痛,为慢性头痛转变机制研究提供了一种较好的实验模型。Objectives： To establish a recurrent headache model in awake behaving rats using inflammato-ry soup to activate dural nociceptors regularly.Methods： Dural inflammation by infusing inflammatory soup（IS） or saline through PE-10 tubing on the dura in male rats were indused.Infusion was repeated once a day for six days.Periorbital von Frey thresholds were tested to monitor the change of trigeminal sensitivi-ty.We observed the ratio of dural mast cells degranulation from SSS stained with toluidine blue were ob-serued.c-Fos immunoreactive positive neurons of once IS infusion group,six times IS and NS infusions group were observed and counted in periagueductal grey matter（PAG） area in coronal brain sections by using standard avidinbiotin immunohistochemistry.Results ： Six infusions of IS induced a decrease in peri-orbital pressure thresholds.The ratio of dural mast cells degranulation from SSS has great difference be-tween IS6 and NS6 infusions group（P〈0.01）.There is a great deference between IS and NS infusions group（P〈0.01）,yet no difference between IS1 infusions group and IS6 infusions group（P〈0.05）.Conclusions： Using inflammatory soup,recurrent headache in rats were indused successfully and provide a better experimental model to elucidate the mechanisms for the transition of episodic to chronic headache.

关 键 词：复方致炎剂 上矢状窦 反复发作性头痛 中脑导水管灰质 c-Fos 

分 类 号：R-332[医药卫生]