二烯丙基三硫化物对大鼠离体肾内动脉血管张力的影响  

Effect of diallyl trisulfide on rat intrarenal arteries in vitro

作　　者：邝素娟[1,2] 邓春玉[1,2] 张光燕[1,2] 饶芳[1,2] 李晓红[1,2] 单志新[1,2] 林秋雄[1,2] 杨敏[1,2] 余细勇[1,2] 

机构地区：[1]广东省人民医院医学研究中心 [2]广东省医学科学院心血管病研究所,广东广州510080

出　　处：《中国药理学通报》2011年第10期1409-1413,共5页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No30900610);广东省自然科学基金资助项目(No10151008002000001);广东省中医药局科研基金资助项目(No2010239);广东省医学科研基金资助项目(NoB2010014);广东省科技计划基金资助项目(No2010B080701090)

摘　　要：目的观察二烯丙基三硫化物(diallyl trisulfide,DATS)对离体肾内动脉张力的影响及其机制。方法 SD大鼠体质量250～300 g,脱臼处死后,显微操作下取出肾内动脉,制备成1.8～2.0 mm长的血管条,每根血管条固定于微血管测定仪的浴槽内,记录张力变化。分别给予血管收缩剂苯肾上腺素(Phe)、血栓素A2受体激动剂(U46619)、5-羟色胺(5-HT)和KCl刺激血管产生持续性收缩,采用累积给药法加入DATS,观察不同浓度的DATS对肾内动脉张力的影响。观察DATS舒张效应的同时用等体积的药物溶剂二甲基亚砜(DMSO)作为对照。结果 DATS能呈浓度依赖性诱导舒张1μmol.L-1 Phe、0.1μmol.L-1 U46619和2μmol.L-1 5-HT预收缩内皮完整的肾内动脉环,pD2分别为(6.06±0.17),(6.14±0.26)和(5.37±0.16),其最大舒张率(Emax)分别为(91.24±2.71)%,(93.44±2.14)%和(92.51±1.15)%;但是,对60 mmol.L-1 KCl预收缩的肾内动脉环无明显舒张作用;分别用eNOS抑制剂L-NAME和sGC抑制剂ODQ预处理内皮完整的肾内动脉环不影响DATS舒张效应;去除肾内动脉血管内皮也不影响DATS对其舒张作用。结论 DATS有浓度依赖性舒张大鼠肾内动脉作用,无明显内皮依赖性。Aim To determine effect of diallyl trisulfide on rat intrarenal arteries in vitro and its mechanism.Methods Sprague-Dawley rats weighing 250~300 g were sacrified by cervical dislocation.The intrarenal arteries were dissected from both kidneys under microscope and cut into two rings segments（1.8~2.0 mm in length）.Each segment was mounted in a multi myograph system,and changes in arterial tension were recorded.1 μmol·L-1 phenylephrine（Phe）,0.1 μmol·L-1 TXA2 receptor agonist 9,11-dideoxy-11a,9a-epoxy-methanoprostaglandin F2a（U46619）,2 μmol·L-1 5-hydroxy tryptamine（5-HT） and 60 mmol·L-1 KCl were used respectively to produce a sustained contraction in renal arteries and then diallyl trisulfide was applied cumulatively.A time-matched vehicle（DMSO） control was performed to ensure a genuine relaxing effect of diallyl trisulfide.Results Diallyl trisulfide was able to induce concentration-dependent relaxations in endothelium-intact rings contracted with Phe,U46619 and 5-HT.pD2 was（6.06 ± 0.17）,（6.14 ± 0.26）and（5.37 ± 0.16）respectively,Emax was（91.24 ± 2.71）%,（93.44 ± 2.14）% and（92.51 ± 1.15）% respectively,but it had no effect on KCl contracted rings with endothelium.Treatment with eNOS inhibitor L-NAME or sGC inhibitor ODQ did not affect the relaxant effect of diallyl trisulfide in rings with endothelium.Endothelium denudation did not affect the diallyl trisulfide relaxant effect yet.Conclusion Diallyl trisulfide has the concentration-dependently relaxant effect on rat intrarenal arteries,which is independent of endothelium.

关 键 词：二烯丙基三硫化物 肾内动脉 血管舒张 内皮 一氧化氮 大鼠 

分 类 号：R-332[医药卫生] R284.1