年龄对小鼠腹腔巨噬细胞TLR2/4表达水平及功能的影响  

Effects of Age on the Expression and Function of TLR2/4 in Murine Peritoneal Macrophages

作　　者：李慧[1,2] 孙颖[1,2] 徐艳[1,2] 王子露[1] 

机构地区：[1]南京医科大学口腔医学研究所,江苏南京210029 [2]南京医科大学附属口腔医院牙周科

出　　处：《口腔医学研究》2011年第9期763-766,共4页Journal of Oral Science Research

基　　金：江苏省高校自然科学研究面上项目(编号:09KJD320001);江苏省自然科学基金(编号:BK2008363)

摘　　要：目的:观察不同年龄组小鼠腹腔巨噬细胞Toll样受体2/4(TLR2/4)表达水平的差别,以及该细胞在脂多糖(LPS)刺激下,炎症因子TNF-α分泌水平的差别。方法:采用real-time PCR和流式细胞技术,检测低龄组和高龄组小鼠腹腔巨噬细胞TLR2/4 mRNA和蛋白表达水平的差异;同时检测1μg/mL大肠杆菌(E.coli)LPS或1mg/L牙龈卟啉单胞菌(P.gingivalis)LPS刺激后,两组细胞TLR2/4表达水平的变化;采用ELISA技术检测炎症因子TNF-α分泌水平的变化。结果:刺激前,两组细胞TLR2/4 mRNA和蛋白表达水平均无明显差别。E.co-li LPS或P.gingivalis LPS刺激后,低龄组细胞TLR2/4mRNA和蛋白表达水平均明显高于高龄组(P<0.05),其分泌的TNF-α水平也显著高于高龄组(P<0.05)。结论:年龄对小鼠腹腔巨噬细胞TLR2/4表达水平没有显著影响,但增龄性变化可能导致TLR2/4功能的减退。Objective： To observe the effect of age on the expression and function of Toll-like receptor 2/4（TLR2/4） in murine peritoneal macrophages.Methods： TLR2/4 mRNA and protein expression were detected in young and aged-murine peritoneal macrophages by real-time PCR and FACS.In addition,TLR2/4 expressions were also determined in these cells stimulated with 1μg/ml E.coli lipopolysaccharides（LPS） or Porphyromonas gingivalis（P.gingivalis） LPS.The levels of TNF-α were investigated by ELISA.Results： There were no significant differences in TLR2/4 mRNA and protein expression in young and aged-murine peritoneal macrophages.However,the higher expressions of TLR2/4 and the increased amounts of TNF-α were observed in young peritoneal macrophages stimulated with 1μg/ml E.coli LPS or P.gingivalis LPS（P0.05）.Conclusion： There were no effects of age on the expression of TLR2/4 in murine peritoneal macrophages,but there were impaired functions of TLR2/4 in aged-mice.

关 键 词：TOLL样受体2 TOLL样受体4 腹腔巨噬细胞 内毒素 

分 类 号：R781.4[医药卫生—口腔医学]