新型根管封闭剂对MC3T3-E1成骨细胞的细胞毒性评估  

Cytotoxicity of New Root Canal Sealers on MC3T3-E1 Osteoblast Cells

作　　者：孙燕[1] 樊明文[1] 李宇红[1] 陆瑶伽[1] 

机构地区：[1]口腔基础医学省部共建国家重点实验室培育基地和口腔生物医学教育部重点实验室.武汉大学口腔医学院儿童牙病科,湖北武汉430079

出　　处：《口腔医学研究》2011年第9期775-777,共3页Journal of Oral Science Research

摘　　要：目的:评估新型根管封闭剂RealSeal sealer、GuttaFlow对MC3T3-E1成骨细胞的细胞毒性,并与传统的AH Plus比较。方法:制备RealSeal sealer、GuttaFlow和AH Plus(固化7d)的材料浸提液,倍比稀释为4种浓度:100%、50%、25%、12.5%;MC3T3-E1细胞于其中分别培养24h和72h,CCK-8法检测细胞存活率,评价不同材料的细胞毒性。结果:在实验期内,RealSeal sealer组的细胞存活率显著低于AH Plus、GuttaFlow(P<0.05),AHPlus、GuttaFlow和阴性对照组间无显著差异。结论:在本实验条件下,RealSeal sealer对MC3T3-E1成骨细胞的毒性最强,而GuttaFlow和AH Plus几乎无细胞毒性。Objective： To investgate the cytotoxicity of new root canal sealers,RealSeal Sealer and GuttaFlow,on MC3T3-E1 osteoblast cells and compare with traditional material AH Plus.Methods： Six samples of each sealer were fabricated in sterile cylindrical Teflon tubes and were kept in a humid chamber at 37℃ for 7 days.Then extraction of the specimens was obtained after incubating with cell culture medium for 3 day.MC3T3-E1 cells were cultured with different concentrations of the extracts（100%,50%,25%,and 12.5%）,and the cell relative proliferation rate was evaluate by CCK-8 assay at 24h and 72 h.Results： RealSeal Sealer showed more cytotoxicity to MC3T3-E1 cells than AH Plus and GuttaFlow（P0.05） within 3 days.The cytotoxicity of GuttaFlow was similar to AH Plus and the negative control.Conclusion： RealSeal Sealer had a strong cytotoxicity on MC3T3-E1cells,while GuttaFlow and AH Plus showed almost nontoxicity.

关 键 词：根管封闭剂 细胞毒性 MC3T3-E1成骨细胞 

分 类 号：R781.33[医药卫生—口腔医学]