贵州地区先天性唇腭裂患者血浆代谢组学的初步研究  

Preliminary Metabonomics Investigation of Plasma from Cleft Lip and/or Palate in Guizhou Province

作　　者：黄光磊[1] 罗洪[1] 

机构地区：[1]贵州省人民医院口腔颌面外科,贵州贵阳550002

出　　处：《口腔医学研究》2011年第9期801-803,807,共4页Journal of Oral Science Research

基　　金：贵州省(2008)筑科农合同第28-2号;社会发展攻关项目

摘　　要：目的:通过检测贵州地区先天性唇腭裂患者血浆内源性代谢产物的变化,探讨基于核磁共振(NMR)的代谢组学技术在临床应用中的可行性。方法:分别收集27例非综合征唇腭裂患者和26例正常人的血浆,采用核磁共振氢谱(H1-NMR)检测血浆代谢谱,并对数据进行主成分分析(PCA),以鉴别血浆代谢产物的变化。结果:非综合征性唇腭裂患者和正常人的血浆代谢产物有明显不同,两组代谢产物各自聚集,分布区域明显分开。先天性唇腭裂患者和正常人的血浆代谢存在差异,至少存在4种不同的代谢产物,分别为天门冬素(Asparagine)、门东氨酸(Aspartic acid)、肌酐(Creatinine)、脯氨酸(Proline)、胆碱(Choline)、酪氨酸(Tyrosine)。非综合征性唇腭裂患者血浆代谢产物以天门冬素、门东氨酸及酪氨酸为主,而正常人血浆代谢产物则以胆碱、肌酐及脯氨酸为主。结论:贵州地区非综合征性唇腭裂患者血浆代谢组学发生变化,同时该技术有望应用于临床。Objective： To access the feasibility of employing nuclear magnetic resonance（NMR）-based metabonomics method in clinical studies,through detecting the change of the endogenous metabolites in plasma from non-syndromic cleft lip and/or palate（NSCL/P） patients and the nol-malformed children in Guizhou province.Methods： Plasma samples of 27 NSCL/P patients and 26 nol-malformed children were collected for clinical biochemical analysis.High-resolution 1H NMR spectroscopy was performed on blood plasma.The metabolic profiles were investigated using pattern recognition techniques,including principal component analysis（PCA）.Results： The PCA plots of the plasma samples presented marked clustering between NSCL/P group and nol-malformed children group.Compared to control group,the level of low molecular metabolites such as Asparagine,Aspartic acid,Tyrosine were higher,while Creatinine,Proline,Choline were lower.Conclusion： Obvious changes in metabonomics of plasma from NSCL/P were observed.The present study clearly demonstrated the great potential of the NMR-based metabonomics approach in clinic diagnosis and screening.

关 键 词：代谢组学 唇腭裂 主成分分析 

分 类 号：R782.2[医药卫生—口腔医学]