牙周膜成纤维细胞对外周血单个核细胞分化的影响  

Effect of Human Periodontal Ligament Fibroblasts on the Differentiation of Peripheral Blood Mononuclear Cells

作　　者：陈建明[1] 兰泽栋[1] 刘嵘[2] 

机构地区：[1]广州医学院口腔医院正畸科,广东广州510140 [2]深圳市儿童医院口腔科

出　　处：《口腔医学研究》2011年第9期808-811,共4页Journal of Oral Science Research

基　　金：广东省社会发展科技计划支助项目(粤科社字[2003]245号)

摘　　要：目的:探讨牙周膜成纤维细胞在破骨细胞形成过程中作用;观察破骨样细胞的生长过程。方法:本实验以含有1α,25(OH)2D3和地塞米松的培养基将牙周膜成纤维细胞、单个核细胞分别进行单独或直接共培养,每3d对TRAP阳性多核破骨细胞的数量及牙本质磨片的吸收陷窝数目和面积分别进行记录、计算。结果:不同时间段间的TRAP阳性单个核细胞与TRAP阳性多核细胞数目相比较,差异具有统计学意义(P<0.05);同时,不同组间的吸收陷窝数目和面积比较,差异具有显著性(P<0.001)。牙周膜成纤维细胞明显增加了共培养组TRAP阳性多核细胞数量、吸收陷窝数目和面积。然而牙周膜成纤维细胞组与单个核细胞组之间的吸收陷窝数目与吸收陷窝面积差异无统计学意义。结论:末梢血单个核细胞需在牙周膜成纤维细胞存在的条件下,才能形成多核的破骨样细胞。同时,在共培养中,可以发现破骨样细胞在体外存活的时间短暂。Objective： To investigate the effect of human periodontal ligament fibroblasts（PDLF） on the formation of osteoclast like cells（OLC） in vitro,and to probe the process of their growth.Methods： Human PDLF and peripheral blood mononuclear cells（PBMC） were co-cultured in the presence of 1α,25（OH）2D3 and dexamethasone for 30 days.Then the numbers of tartrate-resistant acidic phosphatase（TRAP） positive multi-nucleate cells and resorptive pits and the area of resorptive pits were recorded and accumulated every 3 days.Results： There was statistical significant difference in the numbers of TRAP positive multi-nucleate cells（P0.05） at different stages.So were the number of resorptive pits and the area of resorptive pits（P0.001）.And PDLF also significantly increased the number of OLC and resorptive pits and the area of resorptive pits in PDLF-PBMC co-culture（P0.001）.But no statistical significance had been found between PDLF culture and PBMC culture on the number of resorptive pits and the area of resorptive pits.Conclusion： PBMC can differentiate into OLC by co-culture with PDLF.There were no TRAP positive multi-nucleate cells without PDLF.The span of OLC was short in vitro.

关 键 词：破骨样细胞 牙周膜成纤维细胞 共培养 外周血单个核细胞 

分 类 号：R783.5[医药卫生—口腔医学]