Effects of minocycline on the expression of NGF and HSP70 and its neuroprotection role following intracerebral hemorrhage in rats  

作　　者：Jingnan Pu Wei Shi Zizhang Wang Ruizhi Wang Zhenyu Guo Chongxiao Liu Jianjun Sun Ligui Gao Ren Zhou 

机构地区：[1]Department of Neurosurgery, the Second Affiliated Hospital of Medical College of Xi ＇an Jiaotong University, Xi ＇an, Shaanxi 710004, China

出　　处：《The Journal of Biomedical Research》2011年第4期292-298,共7页生物医学研究杂志（英文版）

摘　　要：The present study was aimed to investigate the effects of minocycline （MC） on the expression of nerve growth factor （NGF） and heat shock protein 70 （HSP70） following intracerebral hemorrhage （ICH） in rats, and explore the neuroprotective function of MC. Seventy-eight male SD rats were randomly assigned to three groups： the ICH control group （n = 36）, ICH intervention group （n = 36） and sham operation group （n = 6）. The ICH control group and ICH intervention group were subdivided into 6 subgroups at 1, 2, 4, 5, 7 and 14 d after ICH with 6 rats in each subgroup. Type IV collagenase was injected into the basal nuclei to establish the ICH model. All rats showed symptoms of the nervous system after the model was established, and the sympotsm in the ICH control group were more serious than the ICH intervention group. The number of NGF-positive cells and HSP70-positive cells in the ICH intervention group was higher than that of the ICH control group. MC administration by intraperitoneal injection can increase the expression of NGF and HSP70. MC may inhibit the activation of microglia, the inflammatory reaction and factors, matrix metalloproteinases and apoptosis, thus protecting neurons. The change of the expression of NGF and HSP70 may be involved in the pathway of neuroprotection by MC.The present study was aimed to investigate the effects of minocycline （MC） on the expression of nerve growth factor （NGF） and heat shock protein 70 （HSP70） following intracerebral hemorrhage （ICH） in rats, and explore the neuroprotective function of MC. Seventy-eight male SD rats were randomly assigned to three groups： the ICH control group （n = 36）, ICH intervention group （n = 36） and sham operation group （n = 6）. The ICH control group and ICH intervention group were subdivided into 6 subgroups at 1, 2, 4, 5, 7 and 14 d after ICH with 6 rats in each subgroup. Type IV collagenase was injected into the basal nuclei to establish the ICH model. All rats showed symptoms of the nervous system after the model was established, and the sympotsm in the ICH control group were more serious than the ICH intervention group. The number of NGF-positive cells and HSP70-positive cells in the ICH intervention group was higher than that of the ICH control group. MC administration by intraperitoneal injection can increase the expression of NGF and HSP70. MC may inhibit the activation of microglia, the inflammatory reaction and factors, matrix metalloproteinases and apoptosis, thus protecting neurons. The change of the expression of NGF and HSP70 may be involved in the pathway of neuroprotection by MC.

关 键 词：MINOCYCLINE intracerebral hemorrhage nerve growth factor heat shock protein 70 

分 类 号：Q593.2[生物学—生物化学] Q257