预后良好型肾母细胞瘤p53和低氧诱导因子-1α的表达及与肿瘤侵袭性的关系  

The expressions of p53 and HIF-1α in favorable-histology type of Wilms tumor

作　　者：张丽娟[1] 刘伟[1] 吴荣德[1] 

机构地区：[1]山东大学附属省立医院小儿外科,济南250021

出　　处：《中华小儿外科杂志》2011年第10期764-768,共5页Chinese Journal of Pediatric Surgery

摘　　要：目的 研究小儿预后良好型（FH）肾母细胞瘤（Wilms肿瘤）组织中p53和-1α（HIF-1α）表达水平及其与肿瘤侵袭性的关系.方法 采用免疫组化S-P法检测57例原发Wilms肿瘤组织、21例侵袭灶和10例正常肾脏组织中p53和HIF-1α的表达.结果 p53/HIF-1α在78例Wilms肿瘤组织和10例正常肾脏组织中的阳性表达分别为29.5％（23/78）/39.7％（31/78）和0％/0％,其差异有统计学意义（x2=3.992,P＜0.05;x2=6.136,P＜0.05）.p53蛋白的表达差异在各临床分期间有统计学意义（x2=13.152,P＜0.05）；侵袭组原发灶和非侵袭组的原发灶中p53蛋白阳性率分别为33.3％（7/21）和8.3％（3/36）,两组比较其差别有统计学意义（x2=5.730,P＜0.05）；而HIF-1α在各临床病理因素中的表达差异均无统计学意义（P＞0.05）.p53/HIF-1α蛋白在21例原发灶及其对应的侵袭灶中的表达差异均有统计学意义（Z=-2.093,P＜0.05; Z=-2.723,P＜0.01）.在78例Wilms肿瘤组织中p53和HIF-1α蛋白表达具有正相关性（x2=36.210,P＜0.001,r=0.681）.结论 p53和HIF-1α与Wlims肿瘤的侵袭性相关,这为进一步了解Wilms肿瘤浸润、转移的机制及靶向治疗提供新的思路.Objective To investigate the p53 and HIF-1 α expressions in favorable-histology type of Wilms tumor.Methods The expressions of p53 and HIF-1α were detected in 57 primary Wilms tumors,21 invasive lesions,and 10 normal renal tissues by S-P immunohistochemistry.Results Positive staining of p53 was 29.5% （23/78） in tumors while no positive staining could be found in normal tissues （x2 =3.992,P〈0.05）.The positive staining of HIF-1α was 39.7% （31/78） while no positive staining could be found in normal tissues（x2 =6.136,P〈0.05）.The expression of p53 in different clinic stages had statistical difference （x2 =13.152,P〈0.05）.Positive rate of p53 expression in the primary tumor tissues of invasive group and non-invasive group was 33.3 % （7/21） and 8.3 % （3/36）,respectively （x2 =5.730,P〈0.05）.There was no significant difference between HIF expression and clinopathological factors （P〉0.05）.Significant statistical difference was detected for p53 and HIF-1αexpressions in 21 primary Wilms tumors and matched invasive lesions （Z =- 2.093,P〈0.05/Z =-2.723,P〈0.01）.The expressions of p53 and HIF-1α in 78 Wilms tumor tissues were positively correlated （x2 =36.210,P〈0.001,r=0.681 ）.Conclusions p53 and HIF-1α expressions are correlated with the invasiveness of Wilms tumor.This provides us a new sight into the mechanism of the infiltration and metastases and therapeutic target of the Wilms tumor.

关 键 词：肾母细胞瘤 基因 p53 低氧诱导因子-1 

分 类 号：R737.11[医药卫生—肿瘤]