外源性S100A8在体外抑制HeLa细胞的增殖和侵袭  

Exogenous S100A8 inhibits the proliferation and invasion of HeLa cells in vitro

作　　者：郭元元[1] 游莉[1] 徐兰兰[1] 邹正渝[1] 黎玉叶[1] 孙双双[1] 罗进勇[1] 周兰[1] 

机构地区：[1]重庆医科大学临床检验诊断学教育部重点实验室,重庆400016

出　　处：《基础医学与临床》2011年第10期1082-1087,共6页Basic and Clinical Medicine

基　　金：国家自然科学基金(30772548)

摘　　要：目的探讨外源性S100A8对宫颈癌细胞系HeLa的增殖、凋亡、克隆形成及迁移和侵袭的影响。方法 MTT法检测细胞增殖活性;Hoechst染色检测细胞凋亡;流式细胞技术检测细胞周期;平板集落形成实验检测细胞集落形成;划痕和Transwell侵袭实验分别检测细胞的迁移和侵袭。结果细胞培养3 d时,浓度为100、300和1 000 mg/L的GST-hS100A8组的A值较GST组减少13.64%、19.29%和25.06%(P<0.05);在浓度为100 mg/L时,GST-hS100A8组呈时间依赖性减少(P<0.05);GST-hS100A8组在第3天时细胞凋亡率较GST组增加5.18倍(P<0.05),同时,出现峰值为(19.9±0.76)%的凋亡峰,而对照组没有凋亡峰;GST-hS100A8组的集落形成率较GST组减少30.2%(P<0.005);划痕愈合率较GST组降低30.1%(P<0.05);穿膜细胞数减少48.9%(P<0.05)。结论外源性S100A8可能具有抑制宫颈癌的作用。Objective To study the effect of exogenous S100A8 on cell proliferation,apoptosis,colony formation,migration and invasion of human cervical cancer cell line HeLa.Methods MTT was used to detect the cell proliferation;Hoechst staining was used to detect the cell apoptosis;Flow cytometry was used to detect the variation of cell cycle;colony-forming assay was used to detect the colony formation;Wound healing assay and Transwell chamber experiment were used to detect the cell migration and invasion.Results After treatment with GST-hS100A8 for 3 d,the A value of 100,300 and 1 000 mg/L group of GST-hS100A8 decreased by 13.64%,19.29% and 25.06% compared with GST group,respectively（P0.05）.In the group of 100 mg/L,the A value of GST-hS100A8 decreased in a time-dependent manner（P0.05）.At day 3,cell apoptosis rate in GST-hS100A8 group increased by 5.18 times compared with GST group（P0.05）,Meanwhile,the peak of apoptosis of GST-hS100A8 was（19.9±0.76）%,while the groups of GST and blank had no apoptotic peak;the rate of colony formation of GST-hS100A8 decreased by 30.2%（P0.05） compared with GST group;the healing rate of GSThS100A8 group reduced by 30.1% compared with GST group（P0.05）;the trans-membrane cell number of GST-hS100A8 group reduced by 48.9%（P0.05） compared with GST group.Conclusions Exogenous S100A8 could have the inhibitive effects on cervical cancer.

关 键 词：S100A8 HELA 抑制 

分 类 号：R737.33[医药卫生—肿瘤]