TRAIL诱导Hela细胞凋亡的线粒体信号通路  

TRAIL induced apoptosis in Hela cells by mitochondrial signal pathway

作　　者：叶记林[1] 刘永春[2] 于有江[1] 刘延庆[3] 

机构地区：[1]扬州环境资源职业技术学院医学系,江苏扬州225127 [2]江苏省苏北人民医院,江苏扬州225000 [3]扬州大学医学院,江苏扬州225001

出　　处：《基础医学与临床》2011年第10期1120-1123,共4页Basic and Clinical Medicine

基　　金：扬州环境资源职业技术学院基金(07001)

摘　　要：目的探讨TRAIL诱导人宫颈癌Hela细胞凋亡的线粒体通路。方法琼脂糖凝胶电泳判断细胞凋亡;激光共聚焦、Western blot、荧光免疫和caspase-3活性检测测定细胞线粒体膜电位(MMP)、BCL-2蛋白、细胞色素c(Cyt c)和凋亡诱导因子(AIF)蛋白在细胞中的定位以及caspase-3活性。结果 TRAIL能诱导Hela细胞凋亡,有凋亡细胞特有的DNA梯状条带。同时,TRAIL具有时间依赖性致MMP(P<0.05,P<0.01)减小和BCL-2蛋白含量明显下降,线粒体Cyt c蛋白释放,AIF蛋白向细胞质、细胞核转移,caspase-3活性增强(作用10 h后达到2.25±0.20倍,P<0.05)。结论 TRAIL诱导Hela细胞凋亡途径之一是通过线粒体信号通路进行的。Objective To explore the mitochondrial pathway in the apoptosis of Hela cells induced by TRAIL.Methods Apoptotic cells were detected by DNA electrophoresis.The mitochondria membrane potential（MMP）,the expressions of BCL-2,the location of Cyt c and AIF,the caspase-3 activity were tested by confocal laser microscopy,western blot,immunofluorescence and caspase-3 activity assay.Results TRAIL can induce apoptosis in Hela cells.DNA ladders were showed on agarose gel electrophoresis.At the same time,TRAIL induced the decrease of MMP（P0.05,P0.01） and BCL-2,the release of Cyt c,the translocation of mitochondrial AIF to cytoplasm and nuclei,the increase of caspase-3 activity（the activity increased to 2.25±0.20 fold in 10 h treated cells,P0.05） in the time-dependent manner.Conclusions TRAIL induce the apoptosis of Hela cells through involves mitochondrial signal pathway.

关 键 词：TRAIL HELA细胞 细胞凋亡 线粒体 

分 类 号：R737.33[医药卫生—肿瘤]