消癌平联合奥曲肽对小鼠肝癌移植癌生长抑制作用的研究  

Inhibitory Effect of Xiaoaiping Injection Combined with Octreotide Acetate on Transplanted Live Cancer in Mice

作　　者：王书敏[1] 赵和平[1] 

机构地区：[1]山西医科大学第一临床医学院肿瘤科,太原市030001

出　　处：《中国肿瘤临床》2011年第18期1135-1138,共4页Chinese Journal of Clinical Oncology

基　　金：山西省科技厅攻关项目(编号:20100312029-1)资助~~

摘　　要：目的:观察消癌平注射液联合奥曲肽对H22肝癌的抑制作用,寻找两药联合作用的最适药效浓度,并探讨其可能的作用机制。方法:建立小鼠皮下H22移植癌模型,成瘤后将实验动物随机分为正常组,模型组,消癌平低、中、高剂量组,奥曲肽组,消癌平低、中、高剂量分别联合奥曲肽组。14天后,取脾组织,测脾指数;剥瘤后称重,计算小鼠的肿瘤抑制率;并用ELISA法检测Th1(IL-2、IFN-γ)和Th2(IL-4、IL-10)细胞因子产生水平;免疫组织化学方法测定凋亡蛋白Bcl-2、Bax的表达情况。结果:各治疗组对H22肝癌的生长均有一定的抑制作用,联合用药组的抑瘤作用优于单药组(P<0.05),可见消癌平+奥曲肽联用具有协同作用,其中以高剂量消癌平+奥曲肽的抑瘤作用最好,且联合用药组在提高脾指数、纠正细胞因子的失衡、上调凋亡促进基因Bax的表达、下调凋亡抑制基因Bcl-2的表达方面均优于单药奥曲肽组和同等浓度的单药消癌平组。结论:高剂量消癌平+奥曲肽为抑瘤的最适方案,可在一定程度上促进肿瘤细胞凋亡,提高机体免疫功能。Objective： To observe the inhibitory effect of the combination of xiaoaiping injection and octreotide on H22 tumor-bearing mice, to determine the optimal drug concentrations, and to explore their possible mechanisms of action. Methods： To establish a mice H22 subcutaneous tumor model, the experimental animals were randomly divided into the following model groups after tumor formation： the low-, medium-, and high-dose XAP groups, the OCT group, and the low-, medium-, and high-dose XAP with OCT groups. After 14 days, the splenic tissues were collected to measure the spleen index; the transplanted tumors were excised after treatment and weighted to calculate the tumor growth inhibitory rates; the level of cytokine production of Thl （ IL-2, IFN-γ ） and Th2 （ IL-4, IL-10 ） was detected with the ELISA method; and the expression of apoptosis proteins Bcl-2 and Bax was determined by immunohistochemistry. Results： The growth of the H22 liver cancer cells in the different treatment groups were inhibited by the treatments; the inhibition in the combination groups was better than in the single-agent groups （ P 〈 0.05 ）. Xiaoaiping ＋ octreotide produced a synergistic effect and the group treated with high-dose xiaoaiping ＋ octreotide exhibited the best inhibition rates. The combination group had an increased spleen index, correcting the imbalance in cytokines and upregulating the expression of the apoptosis-promoting gene Bax, downregulating the expression of the apoptosis-inhibiting gene Bcl-2. The effects of the combination treatment were significantly better than those in the single drug group, namely the octreotide monotherapy and the xiaoaiping monotherapy. Conclusion： High-dose xiaoaiping with octreotide is the best concentration for tumor-inhibition. To some extent, the treatment increased the apoptosis rate of the H22 tumor cells in vivo. It also improved the cellular immunity of the mice bearing the H22 cells.

关 键 词：消癌平 奥曲肽 细胞因子 细胞凋亡 

分 类 号：R735.7[医药卫生—肿瘤]