一氧化氮与胆囊收缩素对犬Oddi括约肌的作用  

Effects of nitric oxide and cholecystokinin on the sphincter of Oddi of dogs

作　　者：李甫[1] 范明明[1] 杨超[1] 王婷[1] 张晞文[1] 

机构地区：[1]上海交通大学医学院附属仁济医院普通外科,200001

出　　处：《中华消化外科杂志》2011年第5期380-382,共3页Chinese Journal of Digestive Surgery

基　　金：上海市卫生局科研基金（2010156）

摘　　要：目的探讨一氧化氮和胆囊收缩素（CCK）在犬Oddi括约肌运动调节中的作用。方法测定正常状态及注射CCK、硝普钠、一氧化氮合酶（NOS）抑制剂N-硝基-L-精氨酸甲酯（L—NAME）时，犬Oddi括约肌的基础压、时相收缩频率、时相收缩幅度；免疫组织化学染色法检测犬Oddi括约肌上NOS阳性神经元的表达情况。计量资料的比较采用t检验。结果注射0、20ng／kgCCK后，犬Oddi括约肌基础压、时相收缩频率、时相收缩幅度分别为（27±10）mmHg（1mmHg=0．133kPa）、（10±3）次／min、（32±8）mmHg和（61±14）mmHg、（64±21）次／min、（44±15）mmHg；注射100ng／kgCCK后产生最大抑制作用和最大兴奋作用时，犬Oddi括约肌基础压、时相收缩频率、时相收缩幅度分别为（77±31）mmHg、（69±18）次／min、（79±14）mmHg和（140±21）mmHg、（129±25）次／min、（173±63）mmHg。胆总管滴注硝普钠后，犬Oddi括约肌基础压、时相收缩幅度均下降（t=3．706，5．183，P〈0．05）。而注射L—NAME后，犬Oddi括约肌基础压、时相收缩幅度均升高（t=5．859，3．588，P〈0．05）。结论20ng／kg生理剂量的CCK可舒张犬Oddi括约肌，大剂量CCK可兴奋犬Oddi括约肌；一氧化氮对犬Oddi括约肌发挥舒张效应，此效应可能在CCK的抑制通路中发挥重要作用。Objective To investigate the effects of nitric oxide （NO） and cholecystokinin （CCK） on the regulation of the motility of sphincter of Oddi （ SO ）. Methods The basal pressure, action rate and contraction range of the SO were examined before and after the injection of CCK, sodium nitroprusside （SNP） and NG-nitro- L-argininemethyl ester hydrochloride （L-NAME）. The expression of neurons with positive expression of NO synthase was detected by immunohistochemical staining. The measurement data were analyzed by using the t test. Results The basal pressure, contraction rate and contraction range of the SO were （27± 10） mm Hg （ 1 mm Hg = 0. 133 kPa）, （ 10 ± 3 ） times/minute and （ 32 ± 8） mm Hg before injection of CCK, and （61±14） mm Hg, （ 64 ± 21 ） times/minute, （44± 15 ） mm Hg after injection of CCK of 20 ng/kg. After injection of CCK of 100 ng/kg, the basal pressure, contraction range and contraction rate of the SO were （77 ± 31 ）mm Hg, （69 ± 18 ）times/minutes, （79 ± 14） mm Hg when the inhibition effect of CCK reached peak, and were （ 140 ± 21 ） mm Hg, （ 129 ± 25 ） times/minutes, （173 ± 63 ） mm Hg when the excitatory effect of CCK reached peak. After injection of SNP into the common bile duct, the basal pressure, contraction range and contraction rate of the SO decreased significantly （ t = 3. 706, 5.183, P 〈 0.05 ） , while the 3 indexes increased significantly after injection of SNP （t = 5. 859, 3. 588, P 〈 0.05 ）. Conclusion Different from physiological dose （20 ng/kg） of CCK, large dose of CCK enhances motility of SO intensively. NO relaxes SO, which may play an important role in the inhibitor pathway of CCK.

关 键 词：ODDI括约肌 压力 一氧化氮 胆囊收缩素 犬 

分 类 号：R575[医药卫生—消化系统]