降解毒死蜱多功能菌株的构建及其对土壤的修复作用  

Construction of multifunctional bacterium strain of chlorpyrifos degradation and its bioremediation application

作　　者：李红梅[1] 张广志[1] 张新建[1] 黄玉杰[1] 李纪顺[1] 杨合同[1] 

机构地区：[1]山东省科学院中日友好生物技术研究中心山东省应用微生物重点实验室,山东济南250014

出　　处：《山东科学》2011年第5期14-17,共4页Shandong Science

基　　金：科技部国际合作项目(2010DFA323302;009DFA32340);"十二五"农村领域国家科技计划课题(2011AA10A205)

摘　　要：将实验室保存的有机磷降解酶基因mpd连接到自杀性质粒pUT-Km1上,构建重组质粒pUT-M,通过三亲杂交,将mpd整合到越南伯克氏菌B418的染色体上,获得多功能工程菌株。实验证明工程菌株的原有解磷、解钾、固氮活性没有改变;48 h后对溶液中毒死蜱降解率达到65%;对室内模拟污染土壤有一定的降解能力,20 d对土壤中的毒死蜱降解率达91%。We constructed recombinant plasmid pUT-M by ligating mpd gene on a suicide plasmid pUT-Kml. We integrated mpd gene onto the chromosome of B. vietnamiensis B418 by triparental hybridization and obtained a multifunctional engineering stain. Experimental results show that the engineering stain retains its original activity of phosphorus or potassium decomposition and nitrogen fixation. The strain degrades chlorpyrifos in solution at a rate of 65% 48 hours later. Moreover, the strain can also degrade chlorpyrifos in simulated soil and the degradation rate can reach 9]% 20 days later.

关 键 词：降解毒死蜱 多功能菌株 载体构建 越南伯克氏菌 土壤修复 

分 类 号：X172[环境科学与工程—环境科学]