老年非小细胞肺癌患者Th1/Th2漂移状态与T-bet/GATA3基因表达的关系  

Relationship between Th1/Th2 Drift and T-bet/GATA3 Gene Expression in Elderly Patients with Non-small Cell Lung Cancer

作　　者：陈刚[1] 王清睿[1] 胡少明[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院中西医结合科,武汉430030

出　　处：《华中科技大学学报（医学版）》2011年第5期509-512,共4页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：民政部十一五老年学研究课题资助[No.民人教科字(2007)18-2-39]

摘　　要：目的探讨老年非小细胞肺癌患者Th1/Th2漂移状态及其与T-bet、GATA3基因表达的关系。方法选取老年非小细胞肺癌患者及健康受试者各120例,双抗夹心酶联免疫吸附测定(ELISA)方法检测各组血浆中Th1类细胞因子γ干扰素(IFN-γ)、白细胞介素-2(IL-2)和Th2类细胞因子IL-4、IL-10的浓度;梯度离心法分离出单个核细胞(PB-MC),采用RT-PCR方法检测其T-bet、GATA3mRNA水平。结果老年非小细胞肺癌患者Th1型细胞因子IFN-γ、IL-2水平与老年健康受试者比较明显下降[(10.28±0.88)pg/mL vs(16.94±1.05)pg/mL;(9.06±0.85)pg/mL vs(13.96±0.87)pg/mL)],差异均具有统计学意义;老年非小细胞肺癌患者Th2型细胞因子IL-4、IL-10水平与老年健康受试者比较明显升高[(5.98±0.98)pg/mL vs(3.89±0.28)pg/mL;(47.59±2.89)pg/mL vs(40.47±1.28)pg/mL)],差异均具有统计学意义。老年非小细胞肺癌患者Th1型核转录因子T-bet mRNA的表达水平较老年健康受试者下降[(0.85±0.11)vs(1.46±0.09)];老年非小细胞肺癌患者Th2型核转录因子GATA3mRNA的表达水平较老年健康受试者明显增高[(1.51±0.12)vs(0.89±0.10)],差异均具有统计学意义。结论老年非小细胞肺癌患者存在着Th2漂移现象,该现象可能与影响Th1/Th2的极化状态的关键因子T-bet和GATA3的表达失常有关。Objective To investigate the relationship between Th1/Th2 drift and T-bet/GATA3 gene expression in elderly patients with non-small cell lung cancer（NSCLC）. Methods 120 cases of patients aged over 65 years with NSCLC were enrolled as observation group,and 120 healthy counterparts as control group at the same time.The concentrations of plasma Th1 type cytokines IFN-γ,IL-2 and Th2 type cytokines IL-4,IL-10 were determined by double-antibody sandwich enzyme-linked immunosorbent assay（ELISA）method.The T-bet/GATA3 mRNA levels were detected by RT-PCR method in peripheral blood mononuclear cells（PBMC）isolated by gradient centrifugation. Results The concentrations of Th1 type cytokines IFN-γ and IL-2 in elderly patients with NSCLC were（10.28±0.88） and（9.06±0.85） pg/mL respectively,which was significantly lower than in control group[（16.94±1.05） and（13.96±0.87） pg/mL,P0.05].However,for Th2 type cytokines IL-4 and IL-10,there was the contrary trend.In observation group,IL-4 and IL-10 were significantly higher than in control group[（5.98±0.98） vs（3.89±0.28） and（47.59±2.89） vs（40.47±1.28） pg/mL respectively].Th1-type nuclear transcription factor T-bet mRNA expression level in control group was significantly reduced as compared with that in control group[（0.85±0.11） vs（1.46±0.09）,P0.05].The expression level of GATA3 mRNA was significantly higher in observation group than in control group[（1.51±0.12） vs（0.89±0.10）]. Conclusion There was Th2 drift in elderly patients with NSCLC,which was related with the disorder of T-bet and GATA-3 gene expression.

关 键 词：老年 非小细胞肺癌 TH1/TH2漂移 T-BET GATA3 

分 类 号：R734.2[医药卫生—肿瘤]