c-Ets1对K562细胞BCR-ABL融合基因表达的影响  

Effects of c-Ets1 on BCR-ABL Gene Expression in K562 Cells

作　　者：孟凡凯[1] 孙汉英[1] 李春蕊[1] 刘文励[1] 周剑峰[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院血液内科,武汉430030

出　　处：《华中科技大学学报（医学版）》2011年第5期550-552,共3页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：国家自然科学基金资助项目(No.30570773)

摘　　要：目的探讨转录因子c-Ets1对K562细胞BCR-ABL融合基因表达的影响。方法将c-Ets1基因转染K562细胞,RT-PCR检测转染后BCR-ABL融合基因mRNA的表达;Western blot检测转染后融合蛋白p210的表达;MTT法检测对细胞增殖的影响;生物信息学方法初步分析BCR-ABL启动子的功能。结果 RT-PCR检测表明,c-Ets1可以抑制BCR-ABL基因mRNA的表达;Western blot检测显示c-Ets1可以抑制p210蛋白的表达;转染c-Ets1后细胞的生长抑制率为(25.52±6.75)%;BCR-ABL启动子区存在c-Ets1的潜在作用靶点。结论 c-Ets1可以抑制K562细胞BCR-ABL基因及p210的表达,进而抑制细胞的增殖。Objective To explore the effects of transcription factor c-Ets1 on the BCR-ABL fusion gene expression in K562 cells. Methods After the c-Ets1 gene was transfected into K562 cells,the expression of BCR-ABL gene was detected by RT-PCR and Western blot respectively,the proliferation of K562 cells was measured by MTT methods,and the BCR-ABL promoter region was analyzed by bioinformatics. Results RT-PCR revealed that c-Ets1 could inhibit the expression of BCR-ABL both at mRNA and protein levels.The growth inhibition rate of K562 cells by c-Ets1 was（25.52±6.75）%.Potential targets of c-Ets1 were found in the promoter region of BCR-ABL. Conclusion c-Ets1 can down-regulate the expression of BCR-ABL,which may account for its mechanism in inhibiting the proliferation of K562 cells.

关 键 词：c-Ets1 BCR-ABL 细胞增殖 

分 类 号：R733.72[医药卫生—肿瘤]