大麻受体激动剂对肺癌A549细胞凋亡和增殖的影响  

Effect of Cannabinoid Receptor Activation by THC on Proliferation and Apoptosis of Lung Cancer A549 Cells

作　　者：朱晓琴[1] 胡景鑫[1] 周于婷[2] 白红波[1] 赵青[3] 

机构地区：[1]广州医学院生理学教研室,广州510182 [2]南方医科大学 [3]广州医学院生物化学教研室,广州510182

出　　处：《华中科技大学学报（医学版）》2011年第5期553-557,共5页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：广州市教育局市属高校科技计划(No.08A112);广州市科技计划(No.2010YI-C891);广州医学院博士启动基金(No.2006GD061)资助项目

摘　　要：目的研究大麻受体激动剂(delta9-tetrahydrocannabinol,THC)对肺癌A549细胞凋亡和增殖的影响。方法 MTT法测定THC对A549细胞增殖的影响;苏木精-伊红染色、扫描电镜观察细胞的形态学变化;Western blot法分析大麻受体CB1、CB2的蛋白表达;DNA梯度电泳检测A549细胞凋亡;流式细胞仪分析细胞凋亡率变化。结果 THC预处理后,MTT检测表明THC对A549细胞增殖有明显抑制作用,随着药物浓度增大,抑制作用更加明显;苏木精-伊红染色、扫描电镜观察显示:肺癌A549细胞有典型的细胞凋亡形态;Western blot检测显示:A549细胞大麻受体CB1、CB2水平较正常气道上皮细胞株16HBE升高;DNA梯度电泳法及流式细胞仪检测显示:THC能抑制A549细胞生长,诱导其凋亡,并具有剂量依赖性。结论大麻受体激动剂THC能抑制肺癌细胞的增殖,并诱导肺癌细胞凋亡,此效应可能与大麻受体CB1、CB2作用有关。Objective To study the effect of the cannabinoid receptor activation by THC on the proliferation and apoptosis of lung cancer A549 cells. Methods The effects of THC on proliferation of A549 cells were measured by using MTT assay,and morphological changes of A549 cells after HE staining were observed under an electron microscopy.Protein expression of cannabinoid receptors CB1 and CB2 was detected by using Western blot.Apoptosis of A549 cells was examined by using DNA gradient gel electrophoresis,and the change of apoptosis rate was analyzed by using flow cytometry. Results After pretreatment with THC,MTT assay revealed that THC could significantly suppress proliferation of A549 cells in a dose-dependent manner.HE staining and electron microscopy displayed that A549 cells had the typical apoptotic morphology.Western blot showed that cannabinoid receptors CB1 and CB2 were increased A549 cells as compared with those in normal airway epithelial cells 16HBE.DNA gradient electrophoresis and flow cytometry demonstrated that THC could inhibit the growth of A549 cells,and induce apoptosis in a dose-dependent manner. Conclusion Cannabinoid receptor agonist THC can inhibit the proliferation of lung cancer cells and induce apoptosis,which may be related to the effects of cannabinoid receptors CB1,CB2.

关 键 词：大麻受体激动剂 肺癌A549细胞 细胞增殖 细胞凋亡 

分 类 号：R73[医药卫生—肿瘤]