模拟微重力促进骨髓间充质干细胞神经营养因子的表达  

Modeled-Microgravity Increases the Expression of Neurotrophins in MSCs

作　　者：杨江河[1] 张蓉[2] 李华[1] 修彬华[1] 刘绍明[1] 

机构地区：[1]兰州军区乌鲁木齐总医院神经外科,新疆乌鲁木齐830000 [2]兰州军区乌鲁木齐总医院妇产科,新疆乌鲁木齐830000

出　　处：《现代生物医学进展》2011年第20期3857-3859,3877,共4页Progress in Modern Biomedicine

摘　　要：目的:探究微重力对干细胞向神经元分化的影响,为临床治疗神经退行性疾病提供新的思维和方法。方法:运用流式细胞术鉴定骨髓间充质干细胞及其微重力对凋亡的影响。运用反转录PCR检测微重力干预干细胞48h及72h后神经营养因子BDNF,CNTF,NGF的表达。运用ELISA检测其分泌营养因子的浓度。结果:流式细胞仪检测细胞凋亡,两组之间并无差异。CNTF分泌在微重力干预后有明显增加(p<0.05)。BDNF浓度有轻度增加。结论:模拟微重力可以促进骨髓间充质干细胞神经营养因子的表达。Objective： To investigate the effects of microgravity on the differentiation of MSCs into neurons. Method： Flow cytometry analysis on the cells of passage 2 was used to identify MSCs. RT-PCR was used to detect the neurotrophins expression in MSCs following microgravity stimulation. The neurotrophins levels in MSCs were detected by ELISA. Results： Compared with that in lg group, the expression of CNTFand BDNF after MG stimulation was higher. Conclusion： Modeled-microgravity increased the expression of neurotrophins in MSCs.

关 键 词：微重力 骨髓间充质干细胞 神经营养因子 

分 类 号：Q95-3[生物学—动物学] R741[医药卫生—神经病学与精神病学]