Different Explants of Lilium lancifolium Have Different Potential to Differentiate and Regenerate in Tissue Culture  

不同外植体对宜兴百合快速微繁殖及再生植株的影响(英文)

作　　者：潘佑找[1,2] 赵宇瑛[1] 刘晓玲[1] 柯尊涛[1] 

机构地区：[1]长江大学园艺园林学院,湖北荆州434025 [2]长江大学长江中游湿地农业教育部工程研究中心,湖北荆州434025

出　　处：《Agricultural Science & Technology》2011年第10期1437-1440,共4页农业科学与技术（英文版）

基　　金：Supported by Fund for Scientific Research in Yangtze University(CDKF2283);Program of Engineering Research Center of Wetland Agriculture in the Middle Reaches of the Yangtze River of Ministry of Education~~

摘　　要：[Objective] The aim was to investigate differences in differentiation and regeneration of the explants from different parts of Lilium lancifolium（Yixing Lily） in tissue culture.[Method] The different parts of scale,leaf and root of Yixing Lily were cultured as explants on MS basic medium supplemented with different concentrations of plant growth regulators,so as to compare their capacity to differentiate and regenerate.[Result] The explants had different potential to differentiate（scale root leaf）.The capacity of different scale parts to differentiate was the lower part middle partupper part;the capacity of different leaf parts to differentiate was the leaf base middle part leaf tip;the capacity of different root parts to differentiate was the root base root tip middle part.[Conclusion] Tissue culture could be well applied in propagation of Yixing Lily.[目的]研究宜兴百合组织培养中外植体对愈伤组织及不定芽分化诱导和再生植株的影响。[方法]以宜兴百合的鳞片、叶片、根的不同部位为外植体,以MS基本培养基加不同的植物生长调节剂配比进行组织培养,比较不同外植体及不同部位在培养时对再生植株的影响。[结果]不同外植体不同部位分化能力存在差异,其差异由强到弱依次为:鳞片、根、叶片。同时鳞片不同部位的分化能力由强到弱为:下>中>上,叶片不同部位的分化能力由强到弱为:叶片基部>中部>叶尖,根的不同部位分化能力由强到弱为:根基部>根尖>根中部。[结论]利用组织培养技术进行宜兴百合的繁殖,具有很好的应用价值。

关 键 词：EXPLANT Tissue culture Yixing Lily Adventitious bud Rapid micropropagation REGENERATION 

分 类 号：S567.239[农业科学—中草药栽培] S644.1[农业科学—作物学]