解钾芽孢杆菌的分离·鉴定及其代谢产物分析  

Isolation,Identification of A Potassium-solubilizing Bacteria and Analysis of the Metabolites

作　　者：杨柳[1] 唐旺全 蒋艳[1] 段月鹏[1] 

机构地区：[1]合肥工业大学生物与食品工程学院,安徽合肥230009 [2]马鞍山科邦生态肥有限公司,安徽当涂243100

出　　处：《安徽农业科学》2011年第28期17265-17267,共3页Journal of Anhui Agricultural Sciences

基　　金：合肥工业大学创新基金项目(XS09079)

摘　　要：[目的]分离与鉴定具有解钾能力的芽孢杆菌,并对筛选出的细菌进行代谢产物分析。[方法]从土壤中分离得到1株具有解钾能力且有一定抗逆性的菌株YJ09,对其进行生理生化性质研究、16S rRNA序列分析,并考察解钾菌培养液中的组分及各组分对钾长石的分解作用。[结果]菌株YJ09为地衣芽孢杆菌(Bacillus licheniformis);解钾菌培养液中含有0.54g/L的有机酸、0.413 mg/L的氨基酸和2.80 g/L的多糖,起解钾作用的主要是多糖。[结论]该研究为具有解钾能力的地衣芽孢杆菌在微生物肥料中的应用提供了理论依据。[Objective] The aim was to investigate the isolation and identification of potassium-solubilizing bacteria and analysis of the metabolites.[Method] A potassium-solubilizing bacteria with a certain environment adaptability was isolated from soil,named YJ09.The phenotype,physiological and biochemical characteristics,phylogenetic analysis of 16S rRNA gene sequence and analysis of component relating of potassium solubilizing in broth.[Result]Strain YJ09 was identified as Bacillus licheniformis.There were 0.54 g/L organic acid,0.413 mg/L amino acid and 2.80 g/L polysaccharides and the potassium-solubilizing effect was mainly related with polysaccharide in cultured broth.[Conclusion] The research provides theoretical basis for application of B.licheniformis with potassium solubilizing ability in microbial fertilizer.

关 键 词：解钾菌 分离 鉴定 地衣芽孢杆菌 

分 类 号：Q939.96[生物学—微生物学]