角蛋白厌氧降解复合菌系构建及其在产沼气中的作用  

Building of Anaerobic Microbial Complex for Keratin Degradation and Its Roles in Biogas Fermentation

作　　者：孙颖杰[1] 邓宇[1] 

机构地区：[1]农业部沼气科学研究所,成都610041

出　　处：《中国沼气》2011年第5期11-16,37,共7页China Biogas

摘　　要：采用Hungate厌氧生物技术,通过高温驯化,首次在实验室构建了3个高效厌氧角蛋白降解复合菌系,其中复合菌系M1-18在最适温度和最适pH值分别为55℃时8,.0～8.5条件下,15 h～16 h内可将羽毛完全降解,降解速度比现有文献报道快。初步探讨了角蛋白厌氧降解菌对猪粪,鸡毛等基质发酵产甲烷的影响,实验表明,35℃时,角蛋白厌氧降解菌能提高猪粪蛋白质的降解速度和生成甲烷的生物转化率,对猪粪发酵产甲烷有较好的促进作用;55℃时,角蛋白厌氧降解菌能快速降解难降解的羽毛角蛋白,并提高由羽毛到甲烷的转化率,对含有鸡毛的沼气发酵有明显的增强作用。Three effective anaerobic keratin-degrading microbial complex were built in laboratory through high temperature domestication by Hungate anaerobic biological technology.The optimum temperature of microbial complex M1-18 was 55 ℃ and optimum pH 8.0~8.5,under which the microbial complex M1-18 could fully degrade feathers in 15 h^16 h.It was the fastest comparing with reported data.Effect of keratin-degrading anaerobic microbe on degradation of pig mature,chicken-feather,etc were experimented and discussed. Experimental results showed that keratin-degrading anaerobic microbe could promote methane production from pig mature fermentation under 35 ℃,and could rapidly degrade chicken-feather, enhancing the fermentation containing chicken-feather as substrate under 55 ℃.

关 键 词：厌氧 角蛋白降解菌 复合菌系 沼气发酵 

分 类 号：X172[环境科学与工程—环境科学] S216.4[农业科学—农业机械化工程]