西葫芦叶片叶绿素提取方法的初步研究  

The Preliminary Study on Determination of Chorophll from Summer Squash

作　　者：尹玲[1] 王长林[1] 王迎杰[1] 王艳玲[1] 

机构地区：[1]中国农业科学院蔬菜花卉研究所,北京100081

出　　处：《北方园艺》2011年第12期14-17,共4页Northern Horticulture

基　　金：国家科技支撑计划资助项目(2009BADB8B02);中央级公益性科研院所基本科研业务费专项资助项目(2060302-2-08);农业部园艺作物遗传改良重点开放实验室资助项目

摘　　要：以西葫芦叶片为试材,研究了提取液种类、提取温度、时间及料液比对叶绿素含量的影响。结果表明:提取液以丙酮∶无水乙醇=1∶1混合液的效果最好,总叶绿素含量最高。叶绿素a、b及叶绿素总含量与提取时间和温度有关,随着提取时间的延长,叶绿素a、b及叶绿素总含量都呈现先升后降的变化趋势。提取时间小于24 h时,低温条件下提取效果较好,叶绿素a、b,总含量高于室温。在一定范围内,叶绿素a/b随着料液比的增加而变大。通过正交实验得到提取西葫芦叶绿素的最佳方法为:丙酮∶无水乙醇=1∶1混合液,料液比1∶40,室温提取48 h。提取叶绿素的简便快速方法为:丙酮∶无水乙醇=1∶1混合液,料液比1∶40,低温提取24 h。The influences of the kinds of extracting solvents,extraction temperature,extraction time and ratio of liquid to solidon the content of chorophll from summer squash leaves were studied.The results showed that method of acetone mixed with ethanol（1∶1）was the best of all whose content of chorophll a＋b was the highest.The content of chorophll a,b,a＋b were related with the time and temprareture of extraction.They increased at first and then decreased.When the soking time was less than 24 h,the content of chorophll a,b,a＋b extracted at 4℃ were higher than that in 25℃.When the soking time was more than 48 h,the content of chorophll a,b,a＋b extracted at 4℃ were lower than that in 25℃.Within certain scope,chorophll a/b increased with the rising of the best ratio of material to solvent.An optimum determination method of chorophll optimized by orthogonal experimental design was extracted at 25℃ for 48 h,V（acetone）∶V（ethanol）=1∶1,the ratio of material to solvent=1∶40.A simple and fast determination method of chorophll was extracted at 4℃ for 24 h,V（acetone）∶V（ethanol）=1∶1,the ratio of material to solvent=1∶40.

关 键 词：西葫芦 叶绿素A/B Arnon法 提取 

分 类 号：S642.6[农业科学—蔬菜学]